ArticleJournal of lipid research2010
Improved method for the quantification of lysophospholipids including enol ether species by liquid chromatography-tandem mass spectrometry.
Article in Journal of lipid research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Facile Titrimetric Assay of Lysophosphatidic Acid in Human Serum and Plasma for Ovarian Cancer Detection.Journal of cancer prevention · 2023Article
- Designing Dual Inhibitors of Autotaxin-LPAR GPCR Axis.Molecules (Basel, Switzerland) · 2022Review
- Untargeted Profiling of Bile Acids and Lysophospholipids Identifies the Lipid Signature Associated with Glycemic Outcome in an Obese Non-Diabetic Clinical Cohort.Biomolecules · 2020Article
- Exploring the Lipidome: Current Lipid Extraction Techniques for Mass Spectrometry Analysis.Metabolites · 2020Review
- Article
- Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions.Analytical chemistry · 2018Article
- Selection of internal standards for accurate quantification of complex lipid species in biological extracts by electrospray ionization mass spectrometry-What, how and why?Mass spectrometry reviews · 2017Review
- Hepatocyte ABCA1 Deletion Impairs Liver Insulin Signaling and Lipogenesis.Cell reports · 2017Article
- Lipidomics by ultrahigh performance liquid chromatography-high resolution mass spectrometry and its application to complex biological samples.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2017Article
- Miltefosine: a novel internal standard approach to lysophospholipid quantitation using LC-MS/MS.Analytical and bioanalytical chemistry · 2017Article
- Novel advances in shotgun lipidomics for biology and medicine.Progress in lipid research · 2016Review
- Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- In vitro anti-Plasmodium falciparum properties of the full set of human secreted phospholipases A2.Infection and immunity · 2015Article
- Comprehensive and quantitative analysis of lysophospholipid molecular species present in obese mouse liver by shotgun lipidomics.Analytical chemistry · 2015Article
- Quantitation of phosphatidic acid and lysophosphatidic acid molecular species using hydrophilic interaction liquid chromatography coupled to electrospray ionization high resolution mass spectrometry.Journal of chromatography. A · 2014Article
- Cytochrome P450 CYP78A9 is involved in Arabidopsis reproductive development.Plant physiology · 2013Article
- Lipidomic analysis of bacterial plasmalogens.Folia microbiologica · 2012Review
- Structural elucidation of molecular species of pacific oyster ether amino phospholipids by normal-phase liquid chromatography/negative-ion electrospray ionization and quadrupole/multiple-stage linear ion-trap mass spectrometry.Analytica chimica acta · 2012Article
- Accurate quantification of lipid species by electrospray ionization mass spectrometry - Meet a key challenge in lipidomics.Metabolites · 2011Article
- COPI acts in both vesicular and tubular transport.Nature cell biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
LC/ESI-MS/MS has been previously demonstrated to be a powerful method to detect and quantify molecular species of glycerophospholipids including lysophospholipids. In this study, we provide an improved pre-mass spectrometry lipid extraction procedure that avoids the acid-catalyzed decomposition of plasmenyl phospholipids that is problematic with previously reported methods. We show that the use of lysophospholipid internal standards with perdeuterated fatty acyl chains avoids isobar problems associated with the use of internal standards containing odd carbon number fatty acyl chains. We also show that LC prior to MS is required to avoid numerous problems associated with isobars and with MS in-source decomposition of lysophosphatidylserine. The reported method of using normal phase chromatography/ESI-MS is used to quantify lysophospholipids in serum and to quantify lysophospholipids produced in mammalian cells by human group X secreted phospholipase A(2). The latter shows that group X phospholipase A(2) added exogenously to cells generates a different set of lysophospholipids compared with enzyme produced endogenously in cells, which supports earlier studies showing that this phospholipase A(2) can act on cell membranes prior to externalization from cells.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.