Evidence mapPaperPMID 19760244Full record

ArticleLipids2009

In vitro simultaneous transfer of lipids to HDL in coronary artery disease and in statin treatment.

Ana C Lo Prete, Clederson H Dina, Carolina H Azevedo, Camila G Puk, Neuza H M Lopes, Whady A Hueb, Raul Cavalcante Maranhão

Registry-linked trialOpen access · greenAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Lipids, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05956145 (Effect of Low Dose of Colchicine on Platelet Reactivty in Patients With Chronic Coronary Disease), which is not on this map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
6.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05956145 nacompletednot on this mapstarted 2021, after this paper: background citation

Effect of Low Dose of Colchicine on Platelet Reactivty in Patients With Chronic Coronary Disease

TypeinterventionalSponsorUniversity of Sao PauloRan2021 to 2024Enrolled80ConditionsPlatelet Aggregation, Spontaneous, Coronary Artery DiseaseArmsColchicine 0.5 MG, Placebo
3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 71 citations in OpenAlex.

  1. Trial
  2. Trial
  3. Trial
  4. Trial
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Ana C Lo PreteFaculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
Clederson H Dina
Carolina H Azevedo
Camila G Puk
Neuza H M Lopes
Whady A Hueb
Raul Cavalcante Maranhão
Hospital do Coração · BRUniversidade de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The exchange of lipids with cells and other lipoproteins is a crucial process in HDL metabolism and for HDL antiatherogenic function. Here, we tested a practical method to quantify the simultaneous transfer to HDL of phospholipids, free-cholesterol, esterified cholesterol and triacylglycerols and to verify the lipid transfer in patients with coronary artery disease (CAD) or undergoing statin treatment. Twenty-eight control subjects without CAD, 27 with CAD and 25 CAD patients under simvastatin treatment were studied. Plasma samples were incubated with a donor nanoemulsion prepared by ultrasonication of the constituent lipids and labeled with radioactive lipids; % lipids transferred to HDL were quantified in the HDL-containing supernatant after chemical precipitation of non-HDL fractions and the nanoemulsion. The assay was precise and reproducible. Increase of temperature (4-37 degrees C), of incubation period (5 min to 2 h), of HDL-cholesterol concentration (33-244 mg/dL) and of mass of nanoemulsion lipids (0.075-0.3 mg/microL) resulted in increased lipid transfer from the nanoemulsion to HDL. In contrast, increasing pH (6.5-8.5) and albumin concentration (3.5-7.0 g/dL) did not affect lipid transfer. There was no difference between CAD and control non-CAD with regard to the lipid transfer, but statin treatment reduced the transfer to HDL of all four lipids. The test herein described is a valid and practical tool for exploring an important aspect of HDL metabolism.

Indexed as

AgedCarrier ProteinsCholesterol, HDLCoronary Artery DiseaseFemaleHumansHydroxymethylglutaryl-CoA Reductase InhibitorsLipid MetabolismMaleMiddle AgedCarrier ProteinsCholesterol, HDLHydroxymethylglutaryl-CoA Reductase Inhibitorslipid transfer protein

Identifiers

PMID19760244
OpenAlexW2019182425

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.