ArticleProceedings of the National Academy of Sciences of the United States of America1991
The v-src inducible gene 9E3/pCEF4 is regulated by both its promoter upstream sequence and its 3' untranslated region.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 30 citations in OpenAlex.
- mRNA stability in mammalian cells.Microbiological reviews · 1995Review
- MGSA/GRO transcription is differentially regulated in normal retinal pigment epithelial and melanoma cells.Molecular and cellular biology · 1994Article
- Differential modulation of plasminogen activator gene expression by oncogene-encoded protein tyrosine kinases.Molecular and cellular biology · 1993Article
- Transcriptional downregulation of the retina-specific QR1 gene by pp60v-src and identification of a novel v-src-responsive unit.Molecular and cellular biology · 1993Article
- Regulation of the junB gene by v-src.Molecular and cellular biology · 1992Article
- Transcriptional activation of the CEF-4/9E3 cytokine gene by pp60v-src.Molecular and cellular biology · 1992Article
- v-Src increases diacylglycerol levels via a type D phospholipase-mediated hydrolysis of phosphatidylcholine.Molecular and cellular biology · 1991Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The 9E3/pCEF4 mRNA is strongly induced in Rous sarcoma virus-transformed chicken embryo fibroblasts when compared to untransformed cells. To identify cis-acting transcriptional elements that confer inducibility by v-src, we isolated the 9E3 promoter upstream region. We found that 1.53 kilobases upstream of the transcriptional start site, when placed in front of a reporter gene, conferred a small degree of inducibility by v-src, in both transient and stable transfections. Two potential AP-1 sites were identified in the 9E3 promoter. AP-1 elements have been implicated previously in mediating a transcriptional response to v-src in fibroblast cell lines. These elements alone do not confer a significant inducibility by v-src in primary chicken embryo fibroblasts. Since the 9E3 mRNA is stabilized in transformed cells, we replaced the 3' untranslated region of the reporter gene with the 9E3 3' untranslated region and found this construct to be strongly responsive to stimulation by v-src. In addition, the 9E3 3' untranslated region increased the response to serum and the tumor promoter phorbol 12-myristate 13-acetate. This suggests that a posttranscriptional mechanism plays a major role in the induction of 9E3 expression.
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