ArticleMolecular biology of the cell2010
Janus-faced enzymes yeast Tgl3p and Tgl5p catalyze lipase and acyltransferase reactions.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Peroxisome deficiency impacts metabolites of lysine, lipid, and polyamine metabolism in Saccharomyces cerevisiae.Histochemistry and cell biology · 2026Article
- Deciphering the role of monoacylglycerol lipases (MAGL) under abiotic stress and lipid metabolism in soybean (Glycine max L.).Plant biotechnology journal · 2025Article
- Engineering yeast for tailored fatty acid profiles.Applied microbiology and biotechnology · 2025Review
- Triacylglycerol mobilization underpins mitochondrial stress recovery.Nature cell biology · 2025Article
- Deletion of ORM2 Causes Oleic Acid-Induced Growth Defects in Saccharomyces cerevisiae.Applied biochemistry and biotechnology · 2023Article
- Vitamin B3 Biotech · 2023Article
- Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.Microbial cell factories · 2022Review
- Silencing the triacylglycerol lipase (TGL) gene decreases the number of apyrene sperm and inhibits oviposition in Sitotroga cerealella.Cellular and molecular life sciences : CMLS · 2021Article
- Phosphatidic acid biosynthesis in the model organism yeast Saccharomyces cerevisiae - a survey.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- Phospholipid turnover and acyl chain remodeling in the yeast ER.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Lipid droplet-mediated lipid and protein homeostasis in budding yeast.FEBS letters · 2018Review
- Lipid droplets are central organelles for meiosis II progression during yeast sporulation.Molecular biology of the cell · 2017Article
- Ymr210wp leads to the accumulation of phospholipids and steryl esters in yeast.Bioinformation · 2017Article
- Lipase genes in Mucor circinelloides: identification, sub-cellular location, phylogenetic analysis and expression profiling during growth and lipid accumulation.Journal of industrial microbiology & biotechnology · 2016Article
- Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria.The Journal of biological chemistry · 2016Article
- Molecular characterization of human ABHD2 as TAG lipase and ester hydrolase.Bioscience reports · 2016Article
- Regulation of the yeast triacylglycerol lipases Tgl4p and Tgl5p by the presence/absence of nonpolar lipids.Molecular biology of the cell · 2016Article
- Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism.The Journal of biological chemistry · 2016Article
- Bioinformatics Reveal Five Lineages of Oleosins and the Mechanism of Lineage Evolution Related to Structure/Function from Green Algae to Seed Plants.Plant physiology · 2015Article
- Lipid droplet dynamics in budding yeast.Cellular and molecular life sciences : CMLS · 2015Review
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2 authors.
Funding
Abstract
In the yeast, mobilization of triacylglycerols (TAGs) is facilitated by the three TAG lipases Tgl3p, Tgl4p, and Tgl5p. Motif search analysis, however, indicated that Tgl3p and Tgl5p do not only contain the TAG lipase motif GXSXG but also an H-(X)(4)-D acyltransferase motif. Interestingly, lipid analysis revealed that deletion of TGL3 resulted in a decrease and overexpression of TGL3 in an increase of glycerophospholipids. Similar results were obtained with TGL5. Therefore, we tested purified Tgl3p and Tgl5p for acyltransferase activity. Indeed, both enzymes not only exhibited lipase activity but also catalyzed acylation of lysophosphatidylethanolamine and lysophosphatidic acid, respectively. Experiments using variants of Tgl3p created by site-directed mutagenesis clearly demonstrated that the two enzymatic activities act independently of each other. We also showed that Tgl3p is important for efficient sporulation of yeast cells, but rather through its acyltransferase than lipase activity. In summary, our results demonstrate that yeast Tgl3p and Tgl5p play a dual role in lipid metabolism contributing to both anabolic and catabolic processes.
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