ArticlePPAR research2009
Regulation of Translational Efficiency by Disparate 5' UTRs of PPARgamma Splice Variants.
Article in PPAR research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Regulation of translation by upstream translation initiation codons of surfactant protein A1 splice variants.American journal of physiology. Lung cellular and molecular physiology · 2015Trial
- Casein Kinase 1 and Human Disease: Insights From the Circadian Phosphoswitch.Frontiers in molecular biosciences · 2022Review
- An Upstream Open Reading Frame Represses Translation of Chicken PPARγ Transcript Variant 1.Frontiers in genetics · 2020Article
- Impacts of Alternative Splicing Events on the Differentiation of Adipocytes.International journal of molecular sciences · 2015Review
- Association of the PPAR-γ Gene with Altered Glucose Levels and Psychosis Profile in Schizophrenia Patients Exposed to Antipsychotics.Psychiatry investigation · 2014Article
- Expression of different functional isoforms in haematopoiesis.International journal of hematology · 2014Review
- Effect of NDP-α-MSH on PPAR-γ and -β expression and anti-inflammatory cytokine release in rat astrocytes and microglia.PloS one · 2013Article
- Regulation of eukaryotic gene expression by the untranslated gene regions and other non-coding elements.Cellular and molecular life sciences : CMLS · 2012Review
- Genome-wide data-mining of candidate human splice translational efficiency polymorphisms (STEPs) and an online database.PloS one · 2010Article
- Genome wide analysis of inbred mouse lines identifies a locus containing Ppar-gamma as contributing to enhanced malaria survival.PloS one · 2010Article
- PPARG: Gene Expression Regulation and Next-Generation Sequencing for Unsolved Issues.PPAR research · 2010Article
- Is there a biological basis for treatment of fibrodysplasia ossificans progressiva with rosiglitazone? Potential benefits and undesired effects.PPAR research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The PPAR-gamma gene encodes for at least 7 unique transcripts due to alternative splicing of five exons in the 5'-untranslated region (UTR). The translated region is encoded by exons 1-6, which are identical in all isoforms. This study investigated the role of the 5'-UTR in regulating the efficiency with which the message is translated to protein. A coupled in vitro transcription-translation assay demonstrated that PPAR-gamma1, -gamma2, and -gamma5 are efficiently translated, whereas PPAR-gamma4 and -gamma7 are poorly translated. An in vivo reporter gene assay using each 5'-UTR upstream of the firefly luciferase gene showed that the 5'-UTRs for PPAR-gamma1, -gamma2, and -gamma4 enhanced translation, whereas the 5'-UTRs for PPAR-gamma5 and -gamma7 inhibited translation. Models of RNA secondary structure, obtained by the mfold software, were used to explain the mechanism of regulation by each 5'-UTR. In general, it was found that the translational efficiency was inversely correlated with the stability of the mRNA secondary structure, the presence of base-pairing in the consensus Kozak sequence, the number of start codons in the 5'-UTR, and the length of the 5'-UTR. A better understanding of posttranscriptional regulation of translation will allow modulation of protein levels without altering transcription.
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.