Evidence mapPaperPMID 20031539Full record

ArticleCirculation. Cardiovascular genetics2008

Correlation of peripheral-blood gene expression with the extent of coronary artery stenosis.

James A Wingrove, Susan E Daniels, Amy J Sehnert, Whittemore Tingley, Michael R Elashoff, Steven Rosenberg, Lutz Buellesfeld, Eberhard Grube, L Kristin Newby, Geoffrey S Ginsburg and 1 more

Registry-linked trialOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Circulation. Cardiovascular genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01334684 (White Blood Cells Gene Expression Profiles as a Tool for Predicting Metformin Efficacy in Patients With Type 2 Diabetes Mellitus), which is not on this map. Cited by 103 papers.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01334684 naunknown statusstarted 2011, after this paper: background citation

White Blood Cells Gene Expression Profiles as a Tool for Predicting Metformin Efficacy in Patients With Type 2 Diabetes Mellitus

Ran2011Enrolled100Registered outcomes2Posted comparisons0ConditionsType 2 DiabetesArmsMetformin
Open the trial in the graph
3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 204 citations in OpenAlex.

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43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

James A WingroveCardioDx, Palo Alto, CA, USA.
Susan E Daniels
Amy J Sehnert
Whittemore Tingley
Michael R Elashoff
Steven Rosenberg
Lutz Buellesfeld
Eberhard Grube
L Kristin Newby
Geoffrey S Ginsburg
William E Kraus
Helios Hospital Siegburg · DECardioDx (United States) · USFX Palo Alto Laboratory · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe molecular pathophysiology of coronary artery disease (CAD) includes cytokine release and a localized inflammatory response within the vessel wall. The extent to which CAD and its severity is reflected by gene expression in circulating cells is unknown. METHODS AND

resultsFrom an initial coronary catheterization cohort we identified 41 patients, comprising 27 cases with angiographically significant CAD and 14 controls without coronary stenosis. Whole-genome microarray analysis performed on peripheral-blood mononuclear cells yielded 526 genes with >1.3-fold differential expression (P<0.05) between cases and controls. Real-time polymerase chain reaction on 106 genes (the 50 most significant microarray genes and 56 additional literature genes) in an independent subset of 95 patients (63 cases, 32 controls) from the same cohort yielded 14 genes (P<0.05) that independently discriminated CAD state in a multivariable analysis that included clinical and demographic factors. From an independent second catheterization cohort, 215 patients were selected for real-time polymerase chain reaction-based replication. A case:control subset of 107 patients (86 cases, 21 controls) replicated 11 of the 14 multivariably significant genes from the first cohort. An analysis of the 14 genes in the entire set of 215 patients demonstrated that gene expression was proportional to maximal coronary artery stenosis (P<0.001 by ANOVA).

conclusionsGene expression in peripheral-blood cells reflects the presence and extent of CAD in patients undergoing angiography.

Indexed as

Gene Expression RegulationAdultAgedAged, 80 and overCase-Control StudiesCohort StudiesCoronary StenosisDemographyFemaleGermanyHumansMaleMiddle AgedNorth CarolinaReproducibility of ResultsReverse Transcriptase Polymerase Chain Reaction

Identifiers

PMID20031539
OpenAlexW2548946619

What Socratic holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.