ArticleNucleic acids research2010
Increased origin activity in transformed versus normal cells: identification of novel protein players involved in DNA replication and cellular transformation.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Replication origin flexibility: a tool to mitigate the hazards of excess replication.Trends in genetics : TIG · 2026Review
- Everything in Moderation: Lessons Learned by Exploiting Moderate Replication Stress in Cancer.Cancers · 2019Review
- Article
- Choosing a suitable method for the identification of replication origins in microbial genomes.Frontiers in microbiology · 2015Review
- Ku protein levels, localization and association to replication origins in different stages of breast tumor progression.Journal of Cancer · 2013Article
- Article
- HBx protein of hepatitis B virus promotes reinitiation of DNA replication by regulating expression and intracellular stability of replication licensing factor CDC6.The Journal of biological chemistry · 2012Article
- Altered replication in human cells promotes DMPK (CTG)(n) · (CAG)(n) repeat instability.Molecular and cellular biology · 2012Article
- Initiation of DNA Replication in the Human Genome.Hereditary genetics : current research · 2012Article
- Differential chromatin structure encompassing replication origins in transformed and normal cells.Genes & cancer · 2012Article
- Broader utilization of origins of DNA replication in cancer cell lines along a 78 kb region of human chromosome 2q34.Journal of cellular biochemistry · 2012Article
- Cdc6 expression represses E-cadherin transcription and activates adjacent replication origins.The Journal of cell biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Using libraries of replication origins generated previously, we identified three clones that supported the autonomous replication of their respective plasmids in transformed, but not in normal cells. Assessment of their in vivo replication activity by in situ chromosomal DNA replication assays revealed that the chromosomal loci corresponding to these clones coincided with chromosomal replication origins in all cell lines, which were more active by 2-3-fold in the transformed by comparison to the normal cells. Evaluation of pre-replication complex (pre-RC) protein abundance at these origins in transformed and normal cells by chromatin immunoprecipitation assays, using anti-ORC2, -cdc6 and -cdt1 antibodies, showed that they were bound by these pre-RC proteins in all cell lines, but a 2-3-fold higher abundance was observed in the transformed by comparison to the normal cells. Electrophoretic mobility shift assays (EMSAs) performed on the most efficiently replicating clone, using nuclear extracts from the transformed and normal cells, revealed the presence of a DNA replication complex in transformed cells, which was barely detectable in normal cells. Subsequent supershift EMSAs suggested the presence of transformation-specific complexes. Mass spectrometric analysis of these complexes revealed potential new protein players involved in DNA replication that appear to correlate with cellular transformation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.