ArticleJournal of cell science2010
Dynamic organisation of prolactin gene expression in living pituitary tissue.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Transcription Factor Pit-1 Affects Transcriptional Timing in the Dual-Promoter Human Prolactin Gene.Endocrinology · 2021Article
- Calcium dynamics and chromatin remodelling underlie heterogeneity in prolactin transcription.Journal of molecular endocrinology · 2021Article
- Disentangling juxtacrine from paracrine signalling in dynamic tissue.PLoS computational biology · 2019Article
- Article
- Discontinuous transcription.Nucleus (Austin, Tex.) · 2018Review
- Article
- Fluctuations of pol I and fibrillarin contents of the nucleoli.Nucleus (Austin, Tex.) · 2017Article
- Cell signaling as a cognitive process.The EMBO journal · 2017Review
- A continuum model of transcriptional bursting.eLife · 2016Article
- Role of Estrogen Response Element in the Human Prolactin Gene: Transcriptional Response and Timing.Molecular endocrinology (Baltimore, Md.) · 2016Article
- Article
- A stochastic transcriptional switch model for single cell imaging data.Biostatistics (Oxford, England) · 2015Article
- Interrogation of inhibitor of nuclear factor κB α/nuclear factor κB (IκBα/NF-κB) negative feedback loop dynamics: from single cells to live animals in vivo.The Journal of biological chemistry · 2012Article
- The prolactin gene: a paradigm of tissue-specific gene regulation with complex temporal transcription dynamics.Journal of neuroendocrinology · 2012Review
- Micro-endoscope for in vivo widefield high spatial resolution fluorescent imaging.Biomedical optics express · 2012Article
- Live imaging of nascent RNA dynamics reveals distinct types of transcriptional pulse regulation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Pulsatile patterns of pituitary hormone gene expression change during development.Journal of cell science · 2011Article
- Molecular morphology of pituitary cells, from conventional immunohistochemistry to fluorescein imaging.Molecules (Basel, Switzerland) · 2011Review
- Dynamic analysis of stochastic transcription cycles.PLoS biology · 2011Article
- The living microarray: a high-throughput platform for measuring transcription dynamics in single cells.BMC genomics · 2011Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Gene expression in living cells is highly dynamic, but temporal patterns of gene expression in intact tissues are largely unknown. The mammalian pituitary gland comprises several intermingled cell types, organised as interdigitated networks that interact functionally to generate co-ordinated hormone secretion. Live-cell imaging was used to quantify patterns of reporter gene expression in dispersed lactotrophic cells or intact pituitary tissue from bacterial artificial chromosome (BAC) transgenic rats in which a large prolactin genomic fragment directed expression of luciferase or destabilised enhanced green fluorescent protein (d2EGFP). Prolactin promoter activity in transgenic pituitaries varied with time across different regions of the gland. Although amplitude of transcriptional responses differed, all regions of the gland displayed similar overall patterns of reporter gene expression over a 50-hour period, implying overall co-ordination of cellular behaviour. By contrast, enzymatically dispersed pituitary cell cultures showed unsynchronised fluctuations of promoter activity amongst different cells, suggesting that transcriptional patterns were constrained by tissue architecture. Short-term, high resolution, single cell analyses in prolactin-d2EGFP transgenic pituitary slice preparations showed varying transcriptional patterns with little correlation between adjacent cells. Together, these data suggest that pituitary tissue comprises a series of cell ensembles, which individually display a variety of patterns of short-term stochastic behaviour, but together yield long-range and long-term coordinated behaviour.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.