ArticleThe Journal of biological chemistry2010
Critical requirement of GABPalpha for normal T cell development.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Transcriptional network dynamics in early T cell development.The Journal of experimental medicine · 2024Review
- Exploring the stage-specific roles of Tcf-1 in T cell development and malignancy at single-cell resolution.Cellular & molecular immunology · 2021Article
- STAT3 Is an Upstream Regulator ofInternational journal of molecular sciences · 2021Article
- Article
- Ets transcription factor GABP controls T cell homeostasis and immunity.Nature communications · 2017Article
- The common γ-chain cytokine receptor: tricks-and-treats for T cells.Cellular and molecular life sciences : CMLS · 2016Review
- Article
- MYC and mitochondrial biogenesis.Cold Spring Harbor perspectives in medicine · 2014Review
- NOTCH1-RBPJ complexes drive target gene expression through dynamic interactions with superenhancers.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- PRISM offers a comprehensive genomic approach to transcription factor function prediction.Genome research · 2013Article
- Understanding the role of ETS-mediated gene regulation in complex biological processes.Advances in cancer research · 2013Review
- Functional analysis of transcription factor binding sites in human promoters.Genome biology · 2012Article
- Targeting tetramer-forming GABPβ isoforms impairs self-renewal of hematopoietic and leukemic stem cells.Cell stem cell · 2012Article
- Fidelity of a BAC-EGFP transgene in reporting dynamic expression of IL-7Rα in T cells.Transgenic research · 2012Article
- Genome-wide analysis reveals conserved and divergent features of Notch1/RBPJ binding in human and murine T-lymphoblastic leukemia cells.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- The possible functions of duplicated ets (GGAA) motifs located near transcription start sites of various human genes.Cellular and molecular life sciences : CMLS · 2011Article
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
GA binding protein (GABP) consists of GABPalpha and GABPbeta subunits. GABPalpha is a member of Ets family transcription factors and binds DNA via its conserved Ets domain, whereas GABPbeta does not bind DNA but possesses transactivation activity. In T cells, GABP has been demonstrated to regulate the gene expression of interleukin-7 receptor alpha chain (IL-7Ralpha) and postulated to be critical in T cell development. To directly investigate its function in early thymocyte development, we used GABPalpha conditional knock-out mice where the exons encoding the Ets DNA-binding domain are flanked with LoxP sites. Ablation of GABPalpha with the Lck-Cre transgene greatly diminished thymic cellularity, blocked thymocyte development at the double negative 3 (DN3) stage, and resulted in reduced expression of T cell receptor (TCR) beta chain in DN4 thymocytes. By chromatin immunoprecipitation, we demonstrated in DN thymocytes that GABPalpha is associated with transcription initiation sites of genes encoding key molecules in TCR rearrangements. Among these GABP-associated genes, knockdown of GABPalpha expression by RNA interference diminished expression of DNA ligase IV, Artemis, and Ku80 components in DNA-dependent protein kinase complex. Interestingly, forced expression of prearranged TCR but not IL-7Ralpha can alleviate the DN3 block in GABPalpha-targeted mice. Our observations collectively indicate that in addition to regulating IL-7Ralpha expression, GABP is critically required for TCR rearrangements and hence normal T cell development.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.