ArticleBiochemistry2010
Direct interaction of the mouse cytomegalovirus m152/gp40 immunoevasin with RAE-1 isoforms.
Article in Biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Direct antigen presentation is the canonical pathway of cytomegalovirus CD8 T-cell priming regulated by balanced immune evasion ensuring a strong antiviral response.Frontiers in immunology · 2023Article
- Review
- Hematopoietic cell-mediated dissemination of murine cytomegalovirus is regulated by NK cells and immune evasion.PLoS pathogens · 2021Article
- Modulation of innate and adaptive immunity by cytomegaloviruses.Nature reviews. Immunology · 2020Review
- Parallel Evolution of Chemokine Binding by Structurally Related Herpesvirus Decoy Receptors.Structure (London, England : 1993) · 2016Article
- An endocytic YXXΦ (YRRF) cargo sorting motif in the cytoplasmic tail of murine cytomegalovirus AP2 'adapter adapter' protein m04/gp34 antagonizes virus evasion of natural killer cells.Medical microbiology and immunology · 2015Article
- MCMV avoidance of recognition and control by NK cells.Seminars in immunopathology · 2014Review
- Human herpesvirus 7 U21 tetramerizes to associate with class I major histocompatibility complex molecules.Journal of virology · 2014Article
- The p36 isoform of murine cytomegalovirus m152 protein suffices for mediating innate and adaptive immune evasion.Viruses · 2013Article
- Analytical Ultracentrifugation as a Tool for Studying Protein Interactions.Biophysical reviews · 2013Article
- Overview of current methods in sedimentation velocity and sedimentation equilibrium analytical ultracentrifugation.Current protocols in protein science · 2013Article
- Structural basis of mouse cytomegalovirus m152/gp40 interaction with RAE1γ reveals a paradigm for MHC/MHC interaction in immune evasion.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Global multi-method analysis of affinities and cooperativity in complex systems of macromolecular interactions.Analytical chemistry · 2012Article
- Strategies for assessing proton linkage to bimolecular interactions by global analysis of isothermal titration calorimetry data.The Journal of chemical thermodynamics · 2012Article
- All is fair in virus-host interactions: NK cells and cytomegalovirus.Trends in molecular medicine · 2011Article
- B7-H6/NKp30 interaction: a mechanism of alerting NK cells against tumors.Cellular and molecular life sciences : CMLS · 2011Review
- The analysis of macromolecular interactions by sedimentation equilibrium.Methods (San Diego, Calif.) · 2011Article
- The boundary structure in the analysis of reversibly interacting systems by sedimentation velocity.Methods (San Diego, Calif.) · 2011Article
- Reverse genetics modification of cytomegalovirus antigenicity and immunogenicity by CD8 T-cell epitope deletion and insertion.Journal of biomedicine & biotechnology · 2011Review
- How the virus outsmarts the host: function and structure of cytomegalovirus MHC-I-like molecules in the evasion of natural killer cell surveillance.Journal of biomedicine & biotechnology · 2011Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Cytomegaloviruses (CMVs) are ubiquitous species-specific viruses that establish acute, persistent, and latent infections. Both human and mouse CMVs encode proteins that inhibit the activation of natural killer (NK) cells by downregulating cellular ligands for the NK cell activating receptor, NKG2D. The MCMV glycoprotein m152/gp40 downregulates the surface expression of RAE-1 to prevent NK cell control in vivo. So far, it is unclear if there is a direct interaction between m152 and RAE-1 and, if so, if m152 interacts differentially with the five identified RAE-1 isoforms, which are expressed as two groups in MCMV-susceptible or -resistant mouse strains. To address these questions, we expressed and purified the extracellular domains of RAE-1 and m152 and performed size exclusion chromatography binding assays as well as analytical ultracentrifugation and isothermal titration calorimetry to characterize these interactions quantitatively. We further evaluated the role of full-length and naturally glycosylated m152 and RAE-1 in cotransfected HEK293T cells. Our results confirmed that m152 binds RAE-1 directly, relatively tightly (K(d) < 5 microM), and with 1:1 stoichiometry. The binding is quantitatively different depending on particular RAE-1 isoforms, corresponding to the susceptibility to downregulation by m152. A PLWY motif found in RAE-1beta, although contributing to its affinity for m152, does not influence the affinity of RAE-1gamma or RAE-1delta, suggesting that other differences contribute to the RAE-1-m152 interaction. Molecular modeling of the different RAE-1 isoforms suggests a potential site for the m152 interaction.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.