Trial reportEuropean journal of applied physiology2010
SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: influence of glucose ingestion.
Trial report in European journal of applied physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 2 syntheses or guidelines pooled it, 80 citations in OpenAlex.
- A systematic review and meta-analysis of the SIRT1 response to exercise.Scientific reports · 2023Pooled it
- Factors Influencing AMPK Activation During Cycling Exercise: A Pooled Analysis and Meta-Regression.Sports medicine (Auckland, N.Z.) · 2022Pooled it
- Impact of Exercise Intensity on Systemic Oxidative Stress, Inflammatory Responses, and Sirtuin Levels in Healthy Male Volunteers.International journal of environmental research and public health · 2022Trial
- Effects of Time-Restricted Feeding on Supramaximal Exercise Performance and Body Composition: A Randomized and Counterbalanced Crossover Study in Healthy Men.International journal of environmental research and public health · 2021Trial
- Time-restricted eating and concurrent exercise training reduces fat mass and increases lean mass in overweight and obese adults.Physiological reports · 2021Trial
- Trial
- Exercise training comprising of single 20-s cycle sprints does not provide a sufficient stimulus for improving maximal aerobic capacity in sedentary individuals.European journal of applied physiology · 2016Trial
- Efficacy of reduced exertion high intensity interval training to modify cardiovascular disease-related risk factors-a narrative review.Frontiers in rehabilitation sciences · 2026Review
- Review
- Numerous Trigger-like Interactions of Kinases/Protein Phosphatases in Human Skeletal Muscles Can Underlie Transient Processes in Activation of Signaling Pathways during Exercise.International journal of molecular sciences · 2023Review
- Cellular and Molecular Regulation of Exercise-A Neuronal Perspective.Cellular and molecular neurobiology · 2023Review
- A Potential Role for Sirtuin-1 in Alzheimer's Disease: Reviewing the Biological and Environmental Evidence.Journal of Alzheimer's disease reports · 2023Review
- Hypothalamus-Muscle Parallel Induction of Metabolic Pathways Following Physical Exercise.Frontiers in neuroscience · 2022Article
- Sirtuin 1 is not required for contraction-stimulated glucose uptake in mouse skeletal muscle.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- What Should I Eat before Exercise? Pre-Exercise Nutrition and the Response to Endurance Exercise: Current Prospective and Future Directions.Nutrients · 2020Review
- Proposed Tandem Effect of Physical Activity and Sirtuin 1 and 3 Activation in Regulating Glucose Homeostasis.International journal of molecular sciences · 2019Review
- No effect of meal intake on physiological or perceptual responses to self-selected high intensity interval exercise (HIIE).Biology of sport · 2019Article
- Review
- Exercise and Sirtuins: A Way to Mitochondrial Health in Skeletal Muscle.International journal of molecular sciences · 2019Review
- Nutritional Ketosis and Mitohormesis: Potential Implications for Mitochondrial Function and Human Health.Journal of nutrition and metabolism · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr(172)-AMPK phosphorylation and increased PGC-1alpha mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKalpha phosphorylation. Exercise elicited different patterns of Ser(221)-ACCbeta, Ser(473)-Akt and Thr(642)-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser(485)-AMPKalpha1/Ser(491)-AMPKalpha2 phosphorylation was reduced by 33% coinciding with increased Thr(172)-AMPKalpha phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr(172)-AMPKalpha phosphorylation. Ser(221)-ACCbeta phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr(172)-AMPKalpha and Ser(221)-ACCbeta phosphorylation. Two hours after the sprint, PGC-1alpha protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1alpha protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr(172)-AMPKalpha phosphorylation and its downstream signaling during the recovery period.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.