ArticlePlanta2010
Isolation and identification of cytoskeleton-associated prolamine mRNA binding proteins from developing rice seeds.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Involvement of a rice mutation in storage protein biogenesis in endosperm and its genomic location.Planta · 2024Article
- mRNA Localization to the Endoplasmic Reticulum in Plant Endosperm Cells.International journal of molecular sciences · 2022Review
- The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status.International journal of molecular sciences · 2021Review
- Zipcode RNA-Binding Proteins and Membrane Trafficking Proteins Cooperate to Transport Glutelin mRNAs in Rice Endosperm.The Plant cell · 2020Article
- Protein sorting into protein bodies during barley endosperm development is putatively regulated by cytoskeleton members, MVBs and the HvSNF7s.Scientific reports · 2020Article
- mRNA Localization in Plant Cells.Plant physiology · 2020Review
- Stable native RIP9 complexes associate with C-to-U RNA editing activity, PPRs, RIPs, OZ1, ORRM1 and ISE2.The Plant journal : for cell and molecular biology · 2019Article
- Targeted Endoplasmic Reticulum Localization of Storage Protein mRNAs Requires the RNA-Binding Protein RBP-L.Plant physiology · 2019Article
- Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant.Journal of experimental botany · 2018Article
- RNA-Binding Protein RBP-P Is Required for Glutelin and Prolamine mRNA Localization in Rice Endosperm Cells.The Plant cell · 2018Article
- Multifunctional RNA Binding Protein OsTudor-SN in Storage Protein mRNA Transport and Localization.Plant physiology · 2017Article
- Comprehensive proteomic analysis of developing protein bodies in maize (Zea mays) endosperm provides novel insights into its biogenesis.Journal of experimental botany · 2016Article
- Rice Endosperm Starch Phosphorylase (Pho1) Assembles with Disproportionating Enzyme (Dpe1) to Form a Protein Complex That Enhances Synthesis of Malto-oligosaccharides.The Journal of biological chemistry · 2016Article
- Ectopic expression of amaranth seed storage albumin modulates photoassimilate transport and nutrient acquisition in sweetpotato.Scientific reports · 2016Article
- Deep sequencing transcriptional fingerprinting of rice kernels for dissecting grain quality traits.BMC genomics · 2015Article
- Characterization of RNA binding protein RBP-P reveals a possible role in rice glutelin gene expression and RNA localization.Plant molecular biology · 2014Article
- Multiple RNA binding protein complexes interact with the rice prolamine RNA cis-localization zipcode sequences.Plant physiology · 2014Article
- Genomic era analyses of RNA secondary structure and RNA-binding proteins reveal their significance to post-transcriptional regulation in plants.Plant science : an international journal of experimental plant biology · 2013Review
- Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues.Journal of experimental botany · 2013Article
- EgRBP42 encoding an hnRNP-like RNA-binding protein from Elaeis guineensis Jacq. is responsive to abiotic stresses.Plant cell reports · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
The messenger RNA of the rice seed storage protein prolamine is targeted to the endoplasmic reticulum (ER) membranes surrounding prolamine protein bodies via a mechanism, which is dependent upon both RNA sorting signals and the actin cytoskeleton. In this study we have used an RNA bait corresponding to the previously characterized 5'CDS prolamine cis-localization sequence for the capture of RNA binding proteins (RBPs) from cytoskeleton-enriched fractions of developing rice seed. In comparison to a control RNA, the cis-localization RNA bait sequence led to the capture of a much larger number of proteins, 18 of which have been identified by tandem mass spectrometry. Western blots demonstrate that several of the candidate proteins analyzed to date show good to excellent specificity for binding to cis-localization sequences over the control RNA bait. Temporal expression studies showed that steady state protein levels for one RNA binding protein, RBP-A, paralleled prolamine gene expression. Immunoprecipitation studies showed that RBP-A is bound to prolamine and glutelin RNAs in vivo, supporting a direct role in storage protein gene expression. Using confocal immunofluorescence microscopy, RBP-A was found to be distributed to multiple compartments in the cell. In addition to the nucleus, RBP-A co-localizes with microtubules and is associated with cortical ER membranes. Collectively, these results indicate that employing a combination of in vitro binding and in vivo binding and localization studies is a valid strategy for the identification of putative prolamine mRNA binding proteins, such as RBP-A, which play a role in controlling expression of storage protein mRNAs in the cytoplasm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.