ArticleJournal of virology2010
Effect of ATP binding and hydrolysis on dynamics of canine parvovirus NS1.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 40 citations in OpenAlex.
- Emergence of canine parvovirus-2c in domestic cats and adaptive evolution of the NS1 gene: Molecular epidemiology of feline parvoviruses in Northern Vietnam (2022-2025).Veterinary world · 2026Article
- Molecular Characterization of Helicase and Nuclease Domains in PPV5 NS1 from Mexican Full-Length Sequence.Viruses · 2026Article
- Pathogenicity of a Canine-Derived Feline Panleukopenia Virus in Cats and NS1 Evolution Associated with Adaptation to Dogs.Animals : an open access journal from MDPI · 2026Article
- Genomic Characterization of Clinical Canine Parvovirus Type 2c Infection in Wild Coyotes (Pathogens (Basel, Switzerland) · 2026Article
- Canine parvovirus infection affects host cell nucleolar organization and ribosome biogenesis.Communications biology · 2026Article
- Epidemiological status and genome characteristics of canine parvovirus in China from 2022 to 2024.Archives of virology · 2025Article
- Phylogenetic analysis and molecular structure of NS1 proteins of porcine parvovirus 5 isolates from Mexico.Archives of virology · 2025Article
- Identification of Host-Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells.Microorganisms · 2025Article
- Parvoviruses NS1 oncolytic attributes: mechanistic insights and synergistic anti-tumor therapeutic strategies.Frontiers in microbiology · 2025Review
- First Isolation and Characterization of Feline Panleukopenia Virus From Wild Raccoon Dogs in the Residential Area of Shanghai, China.Veterinary medicine and science · 2024Article
- Molecular Characterization of Feline Parvovirus from Domestic Cats in Henan Province, China from 2020 to 2022.Veterinary sciences · 2024Article
- Novel mutation N588 residue in the NS1 protein of feline parvovirus greatly augments viral replication.Journal of virology · 2024Article
- Genome Sequences of Canine Parvovirus Type 2c Prevalent in Western Mexico.Archives of Razi Institute · 2024Article
- Two novel sites determine genetic relationships between CPV-2 and FPV: an epidemiological survey of canine and feline parvoviruses in Changchun, China (2020).Frontiers in veterinary science · 2024Article
- Molecular characterisation of the current high prevalence of the new CPV-2c variants in the Southern Vietnamese dogs signifies a widespread in the worldwide dog population.Veterinary medicine and science · 2023Article
- Structure and function of the parvoviral NS1 protein: a review.Virus genes · 2023Review
- Prevalence and Characteristics of Canine Parvovirus Type 2 in Henan Province, China.Microbiology spectrum · 2022Article
- Parvovirus nonstructural protein 2 interacts with chromatin-regulating cellular proteins.PLoS pathogens · 2022Article
- Characterization of the VP2 and NS1 genes from canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) in Northern China.Frontiers in veterinary science · 2022Article
- G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The replication protein NS1 is essential for genome replication and protein production in parvoviral infection. Many of its functions, including recognition and site-specific nicking of the viral genome, helicase activity, and transactivation of the viral capsid promoter, are dependent on ATP. An ATP-binding pocket resides in the middle of the modular NS1 protein in a superfamily 3 helicase domain. Here we have identified key ATP-binding amino acid residues in canine parvovirus (CPV) NS1 protein and mutated amino acids from the conserved A motif (K406), B motif (E444 and E445), and positively charged region (R508 and R510). All mutations prevented the formation of infectious viruses. When provided in trans, all except the R508A mutation reduced infectivity in a dominant-negative manner, possibly by hindering genome replication. These results suggest that the conserved R510 residue, but not R508, is the arginine finger sensory element of CPV NS1. Moreover, fluorescence recovery after photobleaching (FRAP), complemented by computer simulations, was used to assess the binding properties of mutated fluorescent fusion proteins. These experiments identified ATP-dependent and -independent binding modes for NS1 in living cells. Only the K406M mutant had a single binding site, which was concluded to indicate ATP-independent binding. Furthermore, our data suggest that DNA binding of NS1 is dependent on its ability to both bind and hydrolyze ATP.
Indexed as
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.