ArticleJournal of bacteriology2010
Vp1659 is a Vibrio parahaemolyticus type III secretion system 1 protein that contributes to translocation of effector proteins needed to induce cytolysis, autophagy, and disruption of actin structure in HeLa cells.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility inPathogens (Basel, Switzerland) · 2022Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- The Antimicrobial Effect ofFrontiers in microbiology · 2022Article
- Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity.Journal of fish diseases · 2021Article
- Manipulation of IRE1-Dependent MAPK Signaling by a Vibrio Agonist-Antagonist Effector Pair.mSystems · 2021Article
- A GntR Family Transcription Factor (VPA1701) for Swarming Motility and Colonization ofPathogens (Basel, Switzerland) · 2019Article
- The Vibrio alginolyticus T3SS effectors, Val1686 and Val1680, induce cell rounding, apoptosis and lysis of fish epithelial cells.Virulence · 2018Article
- How Bacteria Subvert Animal Cell Structure and Function.Annual review of cell and developmental biology · 2016Review
- Polar flagella rotation in Vibrio parahaemolyticus confers resistance to bacteriophage infection.Scientific reports · 2016Article
- VgrG2 of type VI secretion system 2 of Vibrio parahaemolyticus induces autophagy in macrophages.Frontiers in microbiology · 2015Article
- Transcriptional profiling of Vibrio parahaemolyticus exsA reveals a complex activation network for type III secretion.Frontiers in microbiology · 2015Article
- A Vibrio parahaemolyticus T3SS effector mediates pathogenesis by independently enabling intestinal colonization and inhibiting TAK1 activation.Cell reports · 2013Article
- Characterization of a novel zinc transporter ZnuA acquired by Vibrio parahaemolyticus through horizontal gene transfer.Frontiers in cellular and infection microbiology · 2013Article
- Genetic diversity of clinical and environmental Vibrio parahaemolyticus strains from the Pacific Northwest.Applied and environmental microbiology · 2012Article
- Vibrio parahaemolyticus ExsE is requisite for initial adhesion and subsequent type III secretion system 1-dependent autophagy in HeLa cells.Microbiology (Reading, England) · 2012Article
- Identification of potential type III secretion proteins via heterologous expression of Vibrio parahaemolyticus DNA.Applied and environmental microbiology · 2012Article
- Inflammation and disintegration of intestinal villi in an experimental model for Vibrio parahaemolyticus-induced diarrhea.PLoS pathogens · 2012Article
- Multi-Functional Characteristics of the Pseudomonas aeruginosa Type III Needle-Tip Protein, PcrV; Comparison to Orthologs in other Gram-negative Bacteria.Frontiers in microbiology · 2011Article
- Development of two animal models to study the function of Vibrio parahaemolyticus type III secretion systems.Infection and immunity · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio parahaemolyticus harbors two type III secretion systems (T3SSs; T3SS1 and T3SS2), of which T3SS1 is involved in host cell cytotoxicity. T3SS1 expression is positively regulated by ExsA, and it is negatively regulated by ExsD. We compared the secretion profiles of a wild-type strain (NY-4) of V. parahaemolyticus with those of an ExsD deletion mutant (DeltaexsD) and with a strain of NY-4 that overexpresses T3SS1 (NY-4:pexsA). From this comparison, we detected a previously uncharacterized protein, Vp1659, which shares some sequence homology with LcrV from Yersinia. We show that vp1659 expression is positively regulated by ExsA and is negatively regulated by ExsD. Vp1659 is specifically secreted by T3SS1 of V. parahaemolyticus, and Vp1659 is not required for the successful extracellular secretion of another T3SS1 protein, Vp1656. Mechanical fractionation showed that Vp1659 is translocated into HeLa cells in a T3SS1-dependent manner and that deletion of Vp1659 does not prevent VopS from being translocated into HeLa cells during infection. Deletion of vp1659 significantly reduces cytotoxicity when HeLa cells are infected by V. parahaemolyticus, while complementation of the Deltavp1659 strain restores cytotoxicity. Differential staining showed that Vp1659 is required to induce membrane permeability in HeLa cells. We also show evidence that Vp1659 is required for actin rearrangement and the induction of autophagy. On the basis of these data, we conclude that Vp1659 is a T3SS1-associated protein that is a component of the secretion apparatus and that it is necessary for the efficient translocation of effector proteins into epithelial cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.