ArticleCommunicative & integrative biology2010
Probing novel GPCR interactions using a combination of FRET and TIRF.
Article in Communicative & integrative biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed, 13 citations in OpenAlex.
- Detecting and measuring of GPCR signaling - comparison of human induced pluripotent stem cells and immortal cell lines.Frontiers in endocrinology · 2023Review
- Phosphatidylinositol 4,5-bisphosphate (PIPThe Journal of biological chemistry · 2018Article
- Genetically induced dysfunctions of Kir2.1 channels: implications for short QT3 syndrome and autism-epilepsy phenotype.Human molecular genetics · 2014Article
- Contributions of fluorescence techniques to understanding G protein-coupled receptor dimerisation.Biophysical reviews · 2012Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recent work on G-protein coupled receptors (GPCRs) has highlighted the importance of homo- and heterodimerization in all areas of GPCR function, including trafficking, signaling and desensitization. Novel GPCR dimers and even high-order oligomers are constantly being discovered. Advances in techniques such as fluorescent microscopy have improved our ability to detect these interactions. As GPCRs represent the largest class of transmembrane signaling molecules in biology, these new insights into their function could vastly improve our understanding of the complex physiological role GPCRs play in cellular signaling. Utilizing a combination of classic biochemical approaches and newer techniques such as fluorescence resonance energy transfer (FRET) and total internal reflection fluorescence microscopy (TIRF), we recently demonstrated a novel interaction between M(2) muscarinic receptors and GABA(B) receptors. In this addendum, we address technical aspects of combining FRET and TIRF to study GPCR interactions and further discuss the physiological implications of the M(2)-GABA(B) heterodimer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.