ArticleMolecular biology of the cell2010
Vesicle docking to the spindle pole body is necessary to recruit the exocyst during membrane formation in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- A bridge-like lipid transfer protein is critical for generation of invasive stages in malaria parasites.Nature communications · 2026Article
- Roles for the canonical polarity machinery in the de novo establishment of polarity in budding yeast spores.Molecular biology of the cell · 2025Article
- Roles for the canonical polarity machinery in thebioRxiv : the preprint server for biology · 2025Article
- Membrane and organelle rearrangement during ascospore formation in budding yeast.Microbiology and molecular biology reviews : MMBR · 2024Review
- Meiotic Cytokinesis inJournal of fungi (Basel, Switzerland) · 2024Review
- Recruitment of the lipid kinase Mss4 to the meiotic spindle pole promotes prospore membrane formation inMolecular biology of the cell · 2023Article
- Genetic Dissection of Vps13 Regulation in Yeast Using Disease Mutations from Human Orthologs.International journal of molecular sciences · 2021Article
- The Smk1 MAPK and Its Activator, Ssp2, Are Required for Late Prospore Membrane Development in SporulatingJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Saccharomyces spores are born prepolarized to outgrow away from spore-spore connections and penetrate the ascus wall.Yeast (Chichester, England) · 2021Article
- Analysis of the protein composition of the spindle pole body during sporulation in Ashbya gossypii.PloS one · 2019Article
- Dynamic localization of a yeast development-specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation.Molecular biology of the cell · 2017Article
- Determining protein complex structures based on a Bayesian model of in vivo Förster resonance energy transfer (FRET) data.Molecular & cellular proteomics : MCP · 2014Article
- Exorcising the exocyst complex.Traffic (Copenhagen, Denmark) · 2012Review
- Nanospray FAIMS fractionation provides significant increases in proteome coverage of unfractionated complex protein digests.Molecular & cellular proteomics : MCP · 2012Article
- Discovery of Novel DENN Proteins: Implications for the Evolution of Eukaryotic Intracellular Membrane Structures and Human Disease.Frontiers in genetics · 2012Article
- Sporulation in the budding yeast Saccharomyces cerevisiae.Genetics · 2011Review
- An intein with genetically selectable markers provides a new approach to internally label proteins with GFP.BMC biotechnology · 2011Article
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Authors and funding
7 authors.
Funding
Abstract
During meiosis II in Saccharomyces cerevisiae, the cytoplasmic face of the spindle pole body, referred to as the meiosis II outer plaque (MOP), is modified in both composition and structure to become the initiation site for de novo formation of a membrane called the prospore membrane. The MOP serves as a docking complex for precursor vesicles that are targeted to its surface. Using fluorescence resonance energy transfer analysis, the orientation of coiled-coil proteins within the MOP has been determined. The N-termini of two proteins, Mpc54p and Spo21p, were oriented toward the outer surface of the structure. Mutations in the N-terminus of Mpc54p resulted in a unique phenotype: precursor vesicles loosely tethered to the MOP but did not contact its surface. Thus, these mpc54 mutants separate the steps of vesicle association and docking. Using these mpc54 mutants, we determined that recruitment of the Rab GTPase Sec4p, as well as the exocyst components Sec3p and Sec8p, to the precursor vesicles requires vesicle docking to the MOP. This suggests that the MOP promotes membrane formation both by localization of precursor vesicles to a particular site and by recruitment of a second tethering complex, the exocyst, that stimulates downstream events of fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.