ArticleTissue engineering. Part C, Methods2011
Gelatin-based laser direct-write technique for the precise spatial patterning of cells.
Article in Tissue engineering. Part C, Methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 110 citations in OpenAlex.
- Modeling intratumor heterogeneity in breast cancer.Biofabrication · 2024Review
- Recent advances and applications of artificial intelligence in 3D bioprinting.Biophysics reviews · 2024Review
- Review
- Laser Bioprinting of Cells Using UV and Visible Wavelengths: A Comparative DNA Damage Study.Bioengineering (Basel, Switzerland) · 2022Article
- Hybprinting for musculoskeletal tissue engineering.iScience · 2022Review
- (Bio)manufactured Solutions for Treatment of Bone Defects with Emphasis on US-FDA Regulatory Science Perspective.Advanced nanobiomed research · 2022Article
- Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review.Gels (Basel, Switzerland) · 2022Review
- Materials and technical innovations in 3D printing in biomedical applications.Journal of materials chemistry. B · 2020Review
- 3D Printing of Bioceramics for Bone Tissue Engineering.Materials (Basel, Switzerland) · 2019Review
- Skin bioprinting: the future of burn wound reconstruction?Burns & trauma · 2019Review
- Three-Dimensional Printing and Cell Therapy for Wound Repair.Advances in wound care · 2018Review
- Sacrificial-layer free transfer of mammalian cells using near infrared femtosecond laser pulses.PloS one · 2018Article
- Recent trends in bioinks for 3D printing.Biomaterials research · 2018Review
- Concise Review: Bioprinting of Stem Cells for Transplantable Tissue Fabrication.Stem cells translational medicine · 2017Review
- Laser-assisted bioprinting at different wavelengths and pulse durations with a metal dynamic release layer: A parametric study.International journal of bioprinting · 2017Article
- Laser Direct-Write Onto Live Tissues: A Novel Model for Studying Cancer Cell Migration.Journal of cellular physiology · 2016Article
- Microcapsules and 3D customizable shelled microenvironments from laser direct-written microbeads.Biotechnology and bioengineering · 2016Article
- Precise Tuning of Facile One-Pot Gelatin Methacryloyl (GelMA) Synthesis.Scientific reports · 2016Article
- Printing cancer cells into intact microvascular networks: a model for investigating cancer cell dynamics during angiogenesis.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Recent advances in bioprinting and applications for biosensing.Biosensors · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Laser direct-writing provides a method to pattern living cells in vitro, to study various cell-cell interactions, and to build cellular constructs. However, the materials typically used may limit its long-term application. By utilizing gelatin coatings on the print ribbon and growth surface, we developed a new approach for laser cell printing that overcomes the limitations of Matrigel™. Gelatin is free of growth factors and extraneous matrix components that may interfere with cellular processes under investigation. Gelatin-based laser direct-write was able to successfully pattern human dermal fibroblasts with high post-transfer viability (91% ± 3%) and no observed double-strand DNA damage. As seen with atomic force microscopy, gelatin offers a unique benefit in that it is present temporarily to allow cell transfer, but melts and is removed with incubation to reveal the desired application-specific growth surface. This provides unobstructed cellular growth after printing. Monitoring cell location after transfer, we show that melting and removal of gelatin does not affect cellular placement; cells maintained registry within 5.6 ± 2.5 μm to the initial pattern. This study demonstrates the effectiveness of gelatin in laser direct-writing to create spatially precise cell patterns with the potential for applications in tissue engineering, stem cell, and cancer research.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.