ArticlePlanta2010
Virus-induced gene silencing in rice using a vector derived from a DNA virus.
Article in Planta, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The trial behind it
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Who cites it
32 citing papers in PubMed, 100 citations in OpenAlex.
- Functional validation of OsRPM1 as a positive regulator of bacterial blight resistance in rice via virus-induced gene silencing.Folia microbiologica · 2026Article
- Virus-induced gene silencing as a tool for functional genomics in weeds: Challenges and future directions.Genetics and molecular biology · 2026Article
- Establishment of a Homologous Silencing System with Intact-Plant Infiltration and Minimized Operation for Studying Gene Function in Herbaceous Peonies.International journal of molecular sciences · 2024Article
- Cucumber mosaic virus-induced gene and microRNA silencing in water dropwort (Oenanthe javanica (Blume) DC).Plant methods · 2024Article
- Inoculation of Maize with Sugarcane Mosaic Virus Constructs and Application for RNA Interference in Fall Armyworms.Bio-protocol · 2023Article
- Principles and practice of virus induced gene silencing for functional genomics in plants.Virus genes · 2023Review
- Virus-Induced Gene Silencing (VIGS): A Powerful Tool for Crop Improvement and Its Advancement towards Epigenetics.International journal of molecular sciences · 2023Review
- Molecular Characterization and Virus-Induced Gene Silencing of a Collagen Gene,Life (Basel, Switzerland) · 2022Article
- Virus-induced gene silencing for in planta validation of gene function in cucurbits.Plant physiology · 2022Article
- Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.).Plant methods · 2022Article
- RTBV-Based VIGS Vector for Functional Genomics in Rice: Methodology, Advances, Challenges, and Future Implications.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Virus-Induced Gene Silencing in Wheat and Related Monocot Species.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rice aquaporin OsPIP2;2 is a water-transporting facilitator in relevance to drought-tolerant responses.Plant direct · 2021Article
- The titers of rice tungro bacilliform virus dictate the expression levels of genes related to cell wall dynamics in rice plants affected by tungro disease.Archives of virology · 2021Article
- Review
- Dual Activities of Receptor-Like Kinase OsWAKL21.2 Induce Immune Responses.Plant physiology · 2020Article
- Protein expression and gene editing in monocots using foxtail mosaic virus vectors.Plant direct · 2019Article
- Rice aquaporin PIP1;3 and harpin Hpa1 of bacterial blight pathogen cooperate in a type III effector translocation.Journal of experimental botany · 2019Article
- Gene silencing approaches through virus-based vectors: speeding up functional genomics in monocots.Plant molecular biology · 2019Review
- An efficient and improved method for virus-induced gene silencing in sorghum.BMC plant biology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Virus-induced gene silencing (VIGS) is a method of rapid and transient gene silencing in plants using viral vectors. A VIGS vector for gene silencing in rice has been developed from Rice tungro bacilliform virus (RTBV), a rice-infecting virus containing DNA as the genetic material. A full-length RTBV DNA cloned as a partial dimer in a binary plasmid accumulated in rice plants when inoculated through Agrobacterium (agroinoculation) within 2 weeks and produced detectable levels of RTBV coat protein. Deletion of two of the four viral ORFs did not compromise the ability of the cloned RTBV DNA to accumulate in rice plants. To modify the cloned RTBV DNA as a VIGS vector (pRTBV-MVIGS), the tissue-specific RTBV promoter was replaced by the constitutively expressed maize ubiquitin promoter, sequences comprising the tRNA-binding site were incorporated to ensure reverse transcription-mediated replication, sequences to ensure optimal context for translation initiation of the viral genes were added and a multi-cloning site for the ease of cloning DNA fragments was included. The silencing ability of pRTBV-MVIGS was tested using the rice phytoene desaturase (pds) gene on rice. More than half of the agroinoculated rice plants showed white streaks in leaves within 21 days post-inoculation (dpi), which continued to appear in all emerging leaves till approximately 60-70 dpi. Compared to control samples, real-time PCR showed only 10-40% accumulation of pds transcripts in the leaves showing the streaks. This is the first report of the construction of a VIGS vector for rice which can be introduced by agroinoculation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.