Evidence map›Paper›PMID 21069830›Full record

ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2010

Core fucosylation is required for midline patterning during zebrafish development.

Anandita Seth, Quentin J Machingo, Andreas Fritz, Barry D Shur

Open access · greenAbstract read
In one paragraph

Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Article
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  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Anandita SethDepartment of Cell Biology, Emory University, Atlanta, Georgia, USA.
Quentin J Machingo
Andreas Fritz
Barry D Shur
Emory University · US

Funding

SUBSTRATE INTERACTION IN CRANIOFACIAL MORPHOGENESISR01DE007120 · NIDCR · UNIVERSITY OF TEXAS MD ANDERSON CAN CTR · PI SHUR, BARRY D · 1985 to 2009
$2.6M
NIDCR NIH HHS R01 DE007120NIDCR NIH HHS R01 DE17120
6 · The paper itself

Abstract

Complex carbohydrates represent one of the most polymorphic classes of macromolecules, but their functions during embryonic development remain poorly defined. Herein, we show that knockdown of FucT8, the fucosyltransferase responsible for adding an α1,6 fucosyl residue to the core region of N-linked oligosaccharides, results in defective midline patterning during zebrafish development. Reduced FucT8 expression leads to mild cyclopia, small forebrains, U-shaped somites, among other midline patterning defects. One of the principal FucT8 substrates was identified as Apolipoprotein B (ApoB), the major scaffold protein that is responsible for assembly and secretion of lipoprotein particles in vertebrates. In Drosophila, lipoprotein particles are thought to facilitate cell signaling by serving as a transport vehicle for lipid-modified cell signaling proteins, such as hedgehog. In this regard, knockdown of ApoB expression in zebrafish embryos leads to similar midline patterning defects as those seen in FucT8 morphant embryos. Furthermore, preliminary studies suggest that ApoB facilitates Sonic hedgehog signaling during zebrafish development, analogous to the function of lipoprotein particles during hedgehog signaling in Drosophila.

Indexed as

AnimalsAnimals, Genetically ModifiedApolipoproteins BBlotting, WesternBody PatterningFucosyltransferasesHedgehog ProteinsHep G2 CellsHumansImmunohistochemistryIn Situ HybridizationReverse Transcriptase Polymerase Chain ReactionZebrafishZebrafish ProteinsApolipoproteins BFucosyltransferasesHedgehog ProteinsZebrafish Proteins

Identifiers

PMID21069830
PMCPMC2998996
OpenAlexW1983064690

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.