ArticlePloS one2010
Identification of a novel binding partner of phospholipase cβ1: translin-associated factor X.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Genetic inactivation of Translin/Trax RNase disrupts miRNAs, hippocampal synaptic plasticity, and memory.iScience · 2026Article
- Impacts of Globo H Ceramide on Tumor Microenvironment.Advances in experimental medicine and biology · 2026Review
- RNAi Regulator C3PO Promotes Arbovirus Infection in Insect Vectors.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- PLCβ1 by-passes early growth response -1 to induce the differentiation of neuronal cells.Cell death discovery · 2024Article
- Adenosine AJournal of the American Heart Association · 2023Article
- Biochemical characterization of clinically relevant mutations of human Translin.Molecular and cellular biochemistry · 2023Article
- The roles of phospholipase C-β related signals in the proliferation, metastasis and angiogenesis of malignant tumors, and the corresponding protective measures.Frontiers in oncology · 2023Review
- Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis.Journal of biomedical science · 2022Article
- Activation of Gαq sequesters specific transcripts into Ago2 particles.Scientific reports · 2022Article
- Dissecting the conformation of glycans and their interactions with proteins.Journal of biomedical science · 2020Review
- Regulation of bifunctional proteins in cells: Lessons from the phospholipase Cβ/G protein pathway.Protein science : a publication of the Protein Society · 2020Review
- Deletion of the microRNA-degrading nuclease, translin/trax, prevents pathogenic vascular stiffness.American journal of physiology. Heart and circulatory physiology · 2019Article
- The role of phospholipase Cβ on the plasma membrane and in the cytosol: How modular domains enable novel functions.Advances in biological regulation · 2019Article
- Trax: A versatile signaling protein plays key roles in synaptic plasticity and DNA repair.Neurobiology of learning and memory · 2019Review
- Nuclear phospholipase C isoenzyme imbalance leads to pathologies in brain, hematologic, neuromuscular, and fertility disorders.Journal of lipid research · 2019Review
- Phospholipase Cβ1 regulates proliferation of neuronal cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Phospholipase Cβ interacts with cytosolic partners to regulate cell proliferation.Advances in biological regulation · 2018Review
- Regulation of the activity of the promoter of RNA-induced silencing, C3PO.Protein science : a publication of the Protein Society · 2017Article
- Phospholipase C Beta 1: a Candidate Signature Gene for Proneural Subtype High-Grade Glioma.Molecular neurobiology · 2016Article
- Phospholipase Cβ-TRAX Association Is Required for PC12 Cell Differentiation.The Journal of biological chemistry · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian phospholipase Cβ1 (PLCβ1) is activated by the ubiquitous Gα(q) family of G proteins on the surface of the inner leaflet of plasma membrane where it catalyzes the hydrolysis of phosphatidylinositol 4,5 bisphosphate. In general, PLCβ1 is mainly localized on the cytosolic plasma membrane surface, although a substantial fraction is also found in the cytosol and, under some conditions, in the nucleus. The factors that localize PLCβ1in these other compartments are unknown. Here, we identified a novel binding partner, translin-associated factor X (TRAX). TRAX is a cytosolic protein that can transit into the nucleus. In purified form, PLCβ1 binds strongly to TRAX with an affinity that is only ten-fold weaker than its affinity for its functional partner, Gα(q). In solution, TRAX has little effect on the membrane association or the catalytic activity of PLCβ1. However, TRAX directly competes with Gα(q) for PLCβ1 binding, and excess TRAX reverses Gα(q) activation of PLCβ1. In C6 glia cells, endogenous PLCβ1 and TRAX colocalize in the cytosol and the nucleus, but not on the plasma membrane where TRAX is absent. In Neuro2A cells expressing enhanced yellow and cyano fluorescent proteins (i.e., eYFP- PLCβ1 and eCFP-TRAX), Förster resonance energy transfer (FRET) is observed mostly in the cytosol and a small amount is seen in the nucleus. FRET does not occur at the plasma membrane where TRAX is not found. Our studies show that TRAX, localized in the cytosol and nucleus, competes with plasma-membrane bound Gα(q) for PLCβ1 binding thus stabilizing PLCβ1 in other cellular compartments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.