ArticleMethods (San Diego, Calif.)2011
The analysis of macromolecular interactions by sedimentation equilibrium.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- Structural Mechanism of Filamentation Induced Dampening of GTP Inhibition of Glutamate Dehydrogenase.bioRxiv : the preprint server for biology · 2026Article
- Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in human cells.Communications biology · 2023Article
- Computational Assessment of Protein-protein Binding Affinity by Reversely Engineering the Energetics in Protein Complexes.Genomics, proteomics & bioinformatics · 2021Article
- Structures of ISCth4 transpososomes reveal the role of asymmetry in copy-out/paste-in DNA transposition.The EMBO journal · 2021Article
- Computational studies of protein-protein dissociation by statistical potential and coarse-grained simulations: a case study on interactions between colicin E9 endonuclease and immunity proteins.Physical chemistry chemical physics : PCCP · 2019Article
- The Methods Employed in Mass Spectrometric Analysis of Posttranslational Modifications (PTMs) and Protein-Protein Interactions (PPIs).Advances in experimental medicine and biology · 2019Review
- Integrating Structural Information to Study the Dynamics of Protein-Protein Interactions in Cells.Structure (London, England : 1993) · 2018Article
- A structural organization for the Disrupted in Schizophrenia 1 protein, identified by high-throughput screening, reveals distinctly folded regions, which are bisected by mental illness-related mutations.The Journal of biological chemistry · 2017Article
- Predicting Protein-protein Association Rates using Coarse-grained Simulation and Machine Learning.Scientific reports · 2017Article
- Role of E2-RING Interactions in Governing RNF4-Mediated Substrate Ubiquitination.Journal of molecular biology · 2016Article
- On the acquisition and analysis of microscale thermophoresis data.Analytical biochemistry · 2016Article
- Enrichment and characterization of ferritin for nanomaterial applications.Nanotechnology · 2016Article
- Characterising protein/detergent complexes by triple-detection size-exclusion chromatography.Biological procedures online · 2016Article
- Thermodynamic Interrogation of the Assembly of a Viral Genome Packaging Motor Complex.Biophysical journal · 2015Article
- Computing translational diffusion and sedimentation coefficients: an evaluation of experimental data and programs.European biophysics journal : EBJ · 2015Article
- Variable-Field Analytical Ultracentrifugation: I. Time-Optimized Sedimentation Equilibrium.Biophysical journal · 2015Article
- Combining biophysical methods for the analysis of protein complex stoichiometry and affinity in SEDPHAT.Acta crystallographica. Section D, Biological crystallography · 2015Article
- SEDFIT-MSTAR: molecular weight and molecular weight distribution analysis of polymers by sedimentation equilibrium in the ultracentrifuge.The Analyst · 2014Article
- Protein-protein interactions: switch from classical methods to proteomics and bioinformatics-based approaches.Cellular and molecular life sciences : CMLS · 2014Review
- Structure and dynamics of full-length HIV-1 capsid protein in solution.Journal of the American Chemical Society · 2013Article
Corrections and comments
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Authors and funding
1 author.
Funding
Abstract
The study of macromolecular interactions by sedimentation equilibrium is a highly technical method that requires great care in both the experimental design and data analysis. The complexity of the interacting system that can be analyzed is only limited by the ability to deconvolute the exponential contributions of each of the species to the overall concentration gradient. This is achieved in part through the use of multi-signal data collection and the implementation of soft mass conservation. We illustrate the use of these constraints in SEDPHAT through the study of an A+B+B⇌AB+B⇌ABB system and highlight some of the technical challenges that arise. We show that both the multi-signal analysis and mass conservation result in a precise and robust data analysis and discuss improvements that can be obtained through the inclusion of data from other methods such as sedimentation velocity and isothermal titration calorimetry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.