ArticleCurrent chemical genomics2010
A rapid, inexpensive high throughput screen method for neurite outgrowth.
Article in Current chemical genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- M2ara: unraveling metabolomic drug responses in whole-cell MALDI mass spectrometry bioassays.Bioinformatics (Oxford, England) · 2024Article
- Optimization of a human induced pluripotent stem cell-derived sensory neuron model for the in vitro evaluation of taxane-induced neurotoxicity.Scientific reports · 2024Article
- Real-Time Analysis of Neuronal Cell Cultures for CNS Drug Discovery.Brain sciences · 2024Article
- B355252 Suppresses LPS-Induced Neuroinflammation in the Mouse Brain.Brain sciences · 2024Article
- Loss of POGZ alters neural differentiation of human embryonic stem cells.Molecular and cellular neurosciences · 2022Article
- Optimised techniques for high-throughput screening of differentiated SH-SY5Y cells and application for neurite outgrowth assays.Scientific reports · 2021Article
- Novel Concept of Micro Patterned Micro Titer Plates Fabricated via UV-NIL for Automated Neuronal Cell Assay Read-Out.Nanomaterials (Basel, Switzerland) · 2021Article
- Phenoxythiophene sulfonamide compound B355252 protects neuronal cells against glutamate-induced excitotoxicity by attenuating mitochondrial fission and the nuclear translocation of AIF.Experimental and therapeutic medicine · 2021Article
- Trkb-IP3 Pathway Mediating Neuroprotection in Rat Hippocampal Neuronal Cell Culture Following Induction of Kainic Acid.The Malaysian journal of medical sciences : MJMS · 2018Article
- Exercise and BDNF reduce Aβ production by enhancing α-secretase processing of APP.Journal of neurochemistry · 2017Article
- Quantitative assessment of neural outgrowth using spatial light interference microscopy.Journal of biomedical optics · 2017Article
- In vitro models of axon regeneration.Experimental neurology · 2017Review
- The Mineralocorticoid Agonist Fludrocortisone Promotes Survival and Proliferation of Adult Hippocampal Progenitors.Frontiers in endocrinology · 2016Article
- Differential intensity-dependent effects of magnetic stimulation on the longest neurites and shorter dendrites in neuroscreen-1 cells.Journal of neural engineering · 2015Article
- A facile method for simultaneously measuring neuronal cell viability and neurite outgrowth.Current chemical genomics and translational medicine · 2015Article
- A high-content morphological screen identifies novel microRNAs that regulate neuroblastoma cell differentiation.Oncotarget · 2014Article
- Label-free detection of neuronal differentiation in cell populations using high-throughput live-cell imaging of PC12 cells.PloS one · 2013Article
- Analysis of Gene Expression and Neuronal Phenotype in Neuroscreen-1 (NS-1) Cells.International journal of biomedical investigationArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Neurite outgrowth assays are the most common phenotypic screen to assess chemical effects on neuronal cells. Current automated assays involve expensive equipment, lengthy sample preparation and handling, costly reagents and slow rates of data acquisition and analysis. We have developed a high throughput screen (HTS) for neurite outgrowth using a robust neuronal cell model coupled to fast and inexpensive visualization methods, reduced data volume and rapid data analysis. Neuroscreen-1 (NS-1) cell, a subclone of PC12, possessing rapid growth and enhanced sensitivity to NGF was used as a model neuron. This method reduces preparation time by using cells expressing GFP or native cells stained with HCS CellMask(™) Red in a multiplexed 30 min fixation and staining step. A 2x2 camera binning process reduced both image data files and analysis times by 75% and 60% respectively, compared to current protocols. In addition, eliminating autofocus steps during montage generation reduced data collection time. Pharmacological profiles for stimulation and inhibition of neurite outgrowth by NGF and SU6656 were comparable to current standard method utilizing immunofluorescence detection of tubulin. Potentiation of NGF-induced neurite outgrowth by members of a 1,120-member Prestwick compound library as assayed using this method identified six molecules, including etoposide, isoflupredone acetate, fludrocortisone acetate, thioguanosine, oxyphenbutazone and gibberellic acid, that more than doubled the neurite mass primed by 2 ng/ml NGF. This simple procedure represents an important routine approach in high throughput screening of large chemical libraries using the neurite outgrowth phenotype as a measure of the effects of chemical molecules on neuronal cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.