ArticleInvestigative ophthalmology & visual science2011
Mouse conjunctival forniceal gene expression during postnatal development and its regulation by Kruppel-like factor 4.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- Microbial influx during early postnatal life fortifies the ocular surface and guards against allergic eye disease in mice.Communications biology · 2025Article
- PAX6-dependent differentiation landscape of ocular surface epithelium via single-cell RNA sequencing in hiPSC-derived ocular developmental organoid.Communications biology · 2025Article
- Molecular nature of ocular surface barrier function, diseases that affect it, and its relevance for ocular drug delivery.The ocular surface · 2023Review
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- Dupilumab-Associated Mucin Deficiency (DAMD).Translational vision science & technology · 2020Article
- Conjunctival goblet cells: Ocular surface functions, disorders that affect them, and the potential for their regeneration.The ocular surface · 2020Review
- KLF4 Plays an Essential Role in Corneal Epithelial Homeostasis by Promoting Epithelial Cell Fate and Suppressing Epithelial-Mesenchymal Transition.Investigative ophthalmology & visual science · 2017Article
- Preclinical mouse model to monitor live Muc5b-producing conjunctival goblet cell density under pharmacological treatments.PloS one · 2017Article
- Spatiotemporally Regulated Ablation of Klf4 in Adult Mouse Corneal Epithelial Cells Results in Altered Epithelial Cell Identity and Disrupted Homeostasis.Investigative ophthalmology & visual science · 2015Article
- TGFβ signaling inhibits goblet cell differentiation via SPDEF in conjunctival epithelium.Development (Cambridge, England) · 2014Article
- The ocular surface phenotype of Muc5ac and Muc5b null mice.Investigative ophthalmology & visual science · 2014Article
- Tear film mucins: front line defenders of the ocular surface; comparison with airway and gastrointestinal tract mucins.Experimental eye research · 2013Article
- The Ets transcription factor EHF as a regulator of cornea epithelial cell identity.The Journal of biological chemistry · 2013Article
- Regulation of mouse lens maturation and gene expression by Krüppel-like factor 4.Experimental eye research · 2013Article
- Spdef null mice lack conjunctival goblet cells and provide a model of dry eye.The American journal of pathology · 2013Article
- Transcription factors Runx1 to 3 are expressed in the lacrimal gland epithelium and are involved in regulation of gland morphogenesis and regeneration.Investigative ophthalmology & visual science · 2013Article
- Ocular surface development and gene expression.Journal of ophthalmology · 2013Article
- Retinoic acid-related orphan receptor-α is induced in the setting of DNA damage and promotes pulmonary emphysema.American journal of respiratory and critical care medicine · 2012Article
- Critical role of Klf5 in regulating gene expression during post-eyelid opening maturation of mouse corneas.PloS one · 2012Article
- Evaluation of gene expression profiles and pathways underlying postnatal development in mouse sclera.Molecular vision · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo identify the changes in postnatal mouse conjunctival forniceal gene expression and their regulation by Klf4 during the eye-opening stage when the goblet cells first appear.
methodsLaser microdissection (LMD) was used to collect conjunctival forniceal cells from postnatal (PN) day 9, PN14 and PN20 wild-type (WT), and PN14 Klf4-conditional null (Klf4CN) mice, in which goblet cells are absent, developing, present, and missing, respectively. Microarrays were used to compare gene expression among these groups. Expression of selected genes was validated by quantitative RT-PCR, and spatiotemporal expression was assessed by in situ hybridization.
resultsThis study identified 668, 251, 1160, and 139 transcripts that were increased and 492, 377, 1419, and 57 transcripts that were decreased between PN9 and PN14, PN14 and PN20, PN9 and PN20, and PN14 WT and Klf4CN conjunctiva, respectively. Transcripts encoding transcription factors Spdef, FoxA1, and FoxA3 that regulate goblet cell development in other mucosal epithelia, and epithelium-specific Ets (ESE) transcription factor family members were increased during conjunctival development. Components of pathways related to the mesenchymal-epithelial transition, glycoprotein biosynthesis, mucosal immunity, signaling, and endocytic and neural regulation were increased during conjunctival development. Conjunctival Klf4 target genes differed significantly from the previously identified corneal Klf4 target genes, implying tissue-dependent regulatory targets for Klf4.
conclusionsThe changes in gene expression accompanying mouse conjunctival development were identified, and the role of Klf4 in this process was determined. This study provides new probes for examining conjunctival development and function and reveals that the gene regulatory network necessary for goblet cell development is conserved across different mucosal epithelia.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.