ArticleThe Journal of biological chemistry2011
Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Trial
- The role of Fbxo5 in the development of human malignant tumors.American journal of cancer research · 2022Review
- Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division.Trends in cell biology · 2019Review
- APC/C: current understanding and future perspectives.F1000Research · 2019Review
- Article
- CDK1 and PLK1 coordinate the disassembly and reassembly of the nuclear envelope in vertebrate mitosis.Oncotarget · 2018Article
- Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer.BMC cancer · 2017Article
- Hypersensitivity to DNA damage in antephase as a safeguard for genome stability.Nature communications · 2016Article
- Enhanced expression of early mitotic inhibitor-1 predicts a poor prognosis in esophageal squamous cell carcinoma patients.Oncology letters · 2016Article
- Regulation of mRNA translation during mitosis.eLife · 2015Article
- Review
- Spatiotemporal regulation of the anaphase-promoting complex in mitosis.Nature reviews. Molecular cell biology · 2015Review
- Next-generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank.Breast cancer research : BCR · 2014Article
- Article
- Modulation of cell cycle control during oocyte-to-embryo transitions.The EMBO journal · 2013Review
- Electron microscopy structure of human APC/C(CDH1)-EMI1 reveals multimodal mechanism of E3 ligase shutdown.Nature structural & molecular biology · 2013Article
- A prognostic signature of G(2) checkpoint function in melanoma cell lines.Cell cycle (Georgetown, Tex.) · 2013Article
- Article
- Using gene expression data to identify certain gastro-intestinal diseases.Journal of clinical bioinformatics · 2012Article
- Oscillation of APC/C activity during cell cycle arrest promotes centrosome amplification.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell cycle regulation is characterized by alternating activities of cyclin-dependent kinases (CDKs) and of the ubiquitin ligase anaphase promoting complex/cyclosome (APC/C). During S-phase APC/C is inhibited by early mitotic inhibitor 1 (Emi1) to allow the accumulation of cyclins A and B and to prevent re-replication. Emi1 is degraded at prophase by a Plk1-dependent pathway. Recent studies in which the degradation pathway of Emi1 was disrupted have shown that APC/C is activated at mitotic entry despite stabilization of Emi1. These results suggested the possibility of additional mechanisms other than degradation of Emi1, which release APC/C from inhibition by Emi1 upon entry into mitosis. In this study we report one such mechanism, by which the ability of Emi1 to inhibit APC/C is negatively regulated by CDKs. We show that in Plk1-inhibited cells Emi1 is stabilized and phosphorylated, that Emi1 is phosphorylated by CDKs in mitotic but not S-phase cell extracts, and that Emi1 phosphorylation by mitotic cell extracts or purified CDKs markedly reduces the ability of Emi1 to bind and to inhibit APC/C. Finally, we show that the addition of extracts from S-phase cells to extracts from mitotic cells protects Emi1 from CDK-mediated inactivation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.