ArticleInternational journal of nanomedicine2011
Nuclear entry of hyperbranched polylysine nanoparticles into cochlear cells.
Article in International journal of nanomedicine, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 52 citations in OpenAlex.
- Nanomaterials and nanotechnology in otolaryngology: a narrative review.Nanomedicine (London, England) · 2025Review
- The current status of nanotechnological approaches to therapy and drug delivery in otolaryngology: A contemporary review.Laryngoscope investigative otolaryngology · 2022Review
- Nanomaterials for Inner Ear Diseases: Challenges, Limitations and Opportunities.Materials (Basel, Switzerland) · 2022Review
- Application of Nanomedicine in Inner Ear Diseases.Frontiers in bioengineering and biotechnology · 2021Review
- Advances in genome editing for genetic hearing loss.Advanced drug delivery reviews · 2021Review
- Inner Ear Gene Therapies Take Off: Current Promises and Future Challenges.Journal of clinical medicine · 2020Review
- Toward the Optical Cochlear Implant.Cold Spring Harbor perspectives in medicine · 2019Review
- Gene Therapy for Human Sensorineural Hearing Loss.Frontiers in cellular neuroscience · 2019Review
- Genetic Hearing Loss and Gene Therapy.Genomics & informatics · 2018Article
- Cochlear hair cell regeneration after noise-induced hearing loss: Does regeneration follow development?Hearing research · 2017Review
- Inner ear barriers to nanomedicine-augmented drug delivery and imaging.Journal of otology · 2016Review
- Hyaluronan up-regulation is linked to renal dysfunction and hearing loss induced by silver nanoparticles.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
- A novel chitosan-hydrogel-based nanoparticle delivery system for local inner ear application.Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology · 2015Article
- Genetic Polymorphisms Associated with Hearing Threshold Shift in Subjects during First Encounter with Occupational Impulse Noise.PloS one · 2015Article
- Inner ear hair cell regeneration: A look from the past to the future.Neural regeneration research · 2013Review
- Gene transfer in inner ear cells: a challenging race.Gene therapy · 2013Review
- Nanotechnology: toxicologic pathology.Toxicologic pathology · 2013Review
- Cochlear gene therapy.Current opinion in neurology · 2012Review
- Interaction and localization of synthetic nanoparticles in healthy and cystic fibrosis airway epithelial cells: effect of ozone exposure.Journal of aerosol medicine and pulmonary drug delivery · 2012Article
- Filling the silent void: genetic therapies for hearing impairment.Genetics research international · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGene therapy is a potentially effective therapeutic modality for treating sensorineural hearing loss. Nonviral gene delivery vectors are expected to become extremely safe and convenient, and nanoparticles are the most promising types of vectors. However, infrequent nuclear localization in the cochlear cells limits their application for gene therapy. This study aimed to investigate the potential nuclear entry of hyperbranched polylysine nanoparticles (HPNPs) for gene delivery to cochlear targets.
methodsRat primary cochlear cells and cochlear explants generated from newborn rats were treated with different concentrations of HPNPs. For the in vivo study, HPNPs were administered to the rats' round window membranes. Subcellular distribution of HPNPs in different cell populations was observed with confocal microscope 24 hours after administration.
resultsNuclear entry was observed in various cochlear cell types in vitro and in vivo. In the primary cochlear cell culture, concentration-dependent internalization was observed. In the cochlear organotypic culture, abundant HPNPs were found in the modiolus, including the spiral ganglion, organ of Corti, and lateral wall tissues. In the in vivo study, a gradient distribution of HPNPs through different layers of the round window membrane was observed. HPNPs were also distributed in the cells of the middle ear tissue. Additionally, efficient internalization of HPNPs was observed in the organ of Corti and spiral ganglion cells. In primary cochlear cells, HPNPs induced higher transfection efficiency than did Lipofectamine(™).
conclusionThese results suggest that HPNPs are potentially an ideal carrier for gene delivery into the cochlea.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.