Evidence map›Paper›PMID 21485012›Full record

ArticleDevelopmental neurobiology2011

The GTPase Rem2 regulates synapse development and dendritic morphology.

Amy E Ghiretti, Suzanne Paradis

Open access · greenAbstract read
In one paragraph

Article in Developmental neurobiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
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  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Imaging Structural and Functional Dynamics inCold Spring Harbor protocols · 2022
    Article
  11. Experience-Dependent Development of Dendritic Arbors in Mouse Visual Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020
    Article
  12. TDP-43 dysfunction restricts dendritic complexity by inhibiting CREB activation and altering gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  13. Article
  14. Article
  15. Article
  16. Neural architecture: from cells to circuits.Journal of neurophysiology · 2018
    Review
  17. Article
  18. Article
  19. Article
  20. Two Components of Aversive Memory inThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Amy E GhirettiDepartment of Biology and Volen Center for Complex Systems, Brandeis University, Waltham, Massachusetts 02454, USA.
Suzanne Paradis
Brandeis University · US

Funding

NEUROSCIENCE: FROM CHANNELS TO BEHAVIORT32MH019929 · NIMH · BRANDEIS UNIVERSITY · PI GRIFFITH, LESLIE C · 1994 to 2023
$8.5M
Mouse CoreP30NS045713 · NINDS · BRANDEIS UNIVERSITY · PI ROSBASH, MICHAEL · 2003 to 2013
$7.8M
Semaphorin-Dependent GABAergic Synapse Formation: A Novel Approach to Increasing Inhibition in the Intact BrainR01NS065856 · NINDS · BRANDEIS UNIVERSITY · PI PARADIS, SUZANNE · 2010 to 2024
$5.4M
NIMH NIH HHS T32 MH019929NINDS NIH HHS P30 NS045713NINDS NIH HHS P30NS45713NINDS NIH HHS R01 NS065856NINDS NIH HHS R01NS065856
6 · The paper itself

Abstract

Rem2 is a member of the Rad/Rem/Rem2/Gem/Kir subfamily of small Ras-like GTPases that was identified as an important mediator of synapse development. We performed a comprehensive, loss- of-function analysis of Rem2 function in cultured hippocampal neurons using RNAi to substantially decrease Rem2 protein levels. We found that knockdown of Rem2 decreases the density and maturity of dendritic spines, the primary site of excitatory synapses onto pyramidal neurons in the hippocampus. Knockdown of Rem2 also alters the gross morphology of dendritic arborizations, increasing the number of dendritic branches without altering total neurite length. Thus, Rem2 functions to inhibit dendritic branching and promote the development of dendritic spines and excitatory synapses. Interestingly, binding to the calcium-binding protein calmodulin is required for the Rem2 regulation of dendritic branching. However, this interaction is completely dispensable for synapse development. Overall, our results suggest that Rem2 regulates dendritic branching and synapse development via distinct and overlapping signal transduction pathways.

Indexed as

AnimalsAstrocytesCell LineCell ShapeCoculture TechniquesDendritic SpinesHumansImage Processing, Computer-AssistedImmunohistochemistryMicroscopy, ConfocalMonomeric GTP-Binding ProteinsNeurogenesisNeuronsRatsSignal TransductionSynapsesMonomeric GTP-Binding ProteinsREM2 protein, humanRem2 protein, rat

Identifiers

PMID21485012
PMCPMC3170433
OpenAlexW2000761817

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.