ArticleBMC biochemistry2011
Tyrosine phosphorylation of Munc18c on residue 521 abrogates binding to Syntaxin 4.
Article in BMC biochemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Glucose Transporters: Structure, Trafficking, Physiology, and Disease Relevance.Current issues in molecular biology · 2026Review
- GLUT4 Trafficking and Storage Vesicles: Molecular Architecture, Regulatory Networks, and Their Disruption in Insulin Resistance.International journal of molecular sciences · 2025Review
- Phosphorylation of Syntaxin 4 by the Insulin Receptor Drives Exocytic SNARE Complex Formation to Deliver GLUT4 to the Cell Surface.Biomolecules · 2023Article
- Article
- GLUT4 On the move.The Biochemical journal · 2022Article
- Exocytosis Proteins: Typical and Atypical Mechanisms of Action in Skeletal Muscle.Frontiers in endocrinology · 2022Review
- Structure, function and regulation of mammalian glucose transporters of the SLC2 family.Pflugers Archiv : European journal of physiology · 2020Review
- Insulin regulates astrocyte gliotransmission and modulates behavior.The Journal of clinical investigation · 2018Article
- Exocytosis proteins as novel targets for diabetes prevention and/or remediation?American journal of physiology. Regulatory, integrative and comparative physiology · 2017Review
- Spatiotemporal Regulators for Insulin-Stimulated GLUT4 Vesicle Exocytosis.Journal of diabetes research · 2017Review
- Alternative routes to the cell surface underpin insulin-regulated membrane trafficking of GLUT4.Journal of cell science · 2015Article
- Munc18c: a controversial regulator of peripheral insulin action.Trends in endocrinology and metabolism: TEM · 2014Review
- A phosphotyrosine switch for cargo sequestration at clathrin-coated buds.The Journal of biological chemistry · 2014Article
- Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.Molecular and cellular biology · 2014Article
- Impaired tethering and fusion of GLUT4 vesicles in insulin-resistant human adipose cells.Diabetes · 2013Article
- Regulation of the SNARE-interacting protein Munc18c tyrosine phosphorylation in adipocytes by protein-tyrosine phosphatase 1B.Cell communication and signaling : CCS · 2013Article
- Specialized sorting of GLUT4 and its recruitment to the cell surface are independently regulated by distinct Rabs.Molecular biology of the cell · 2013Article
- Munc18c in adipose tissue is downregulated in obesity and is associated with insulin.PloS one · 2013Article
- GLUT4 exocytosis.Journal of cell science · 2011Review
- mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes.Communicative & integrative biologyArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInsulin stimulates exocytosis of GLUT4 from an intracellular store to the cell surface of fat and muscle cells. Fusion of GLUT4-containing vesicles with the plasma membrane requires the SNARE proteins Syntaxin 4, VAMP2 and the regulatory Sec1/Munc18 protein, Munc18c. Syntaxin 4 and Munc18c form a complex that is disrupted upon insulin treatment of adipocytes. Munc18c is tyrosine phosphorylated in response to insulin in these cells. Here, we directly test the hypothesis that tyrosine phosphorylation of Munc18c is responsible for the observed insulin-dependent abrogation of binding between Munc18c and Syntaxin 4.
resultsWe show that Munc18c is directly phosphorylated by recombinant insulin receptor tyrosine kinase in vitro. Using pull-down assays, we show that phosphorylation abrogates binding of Munc18c to both Syntaxin 4 and the v-SNARE VAMP2, as does the introduction of a phosphomimetic mutation into Munc18c (Y521E).
conclusionOur data indicate that insulin-stimulated tyrosine phosphorylation of Munc18c impairs the ability of Munc18c to bind its cognate SNARE proteins, and may therefore represent a regulatory step in GLUT4 traffic.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.