ArticlePloS one2011
Pancreatic beta cells synthesize neuropeptide Y and can rapidly release peptide co-transmitters.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Pancreatic β-cell regeneration in situ by the ALK3 agonist THR-123.Nature communications · 2025Article
- Single-Vesicle Microelectroanalysis Reveals the Role of PIP2 Phospholipid in Vesicle Opening Dynamics and Its Potential Role in Exocytosis.ACS omega · 2025Article
- Cargo receptor Surf4 regulates endoplasmic reticulum export of proinsulin in pancreatic β-cells.Communications biology · 2022Article
- Distinct Pattern of NPY in Gastro-Entero-Pancreatic System of Goat Kids Fed with a New Standardized Red Orange and Lemon Extract (RLE).Animals : an open access journal from MDPI · 2021Article
- β-Cell Maturation and Identity in Health and Disease.International journal of molecular sciences · 2019Review
- Nonclassical Islet Peptides: Pancreatic and Extrapancreatic Actions.Clinical medicine insights. Endocrinology and diabetes · 2019Review
- Monitoring real-time hormone release kinetics via high-content 3-D imaging of compensatory endocytosis.Lab on a chip · 2018Article
- Confocal Imaging of Neuropeptide Y-pHluorin: A Technique to Visualize Insulin Granule Exocytosis in Intact Murine and Human Islets.Journal of visualized experiments : JoVE · 2017Article
- Neuropeptide Y expression marks partially differentiated β cells in mice and humans.JCI insight · 2017Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Article
- DPP4 in Diabetes.Frontiers in immunology · 2015Review
- Pancreatic islet neuropeptide Y overexpression has minimal effect on islet morphology and β-cell adaptation to high-fat diet.Endocrinology · 2014Article
- PDX1 in ducts is not required for postnatal formation of β-cells but is necessary for their subsequent maturation.Diabetes · 2013Article
- Neuropeptide y gates a stress-induced, long-lasting plasticity in the sympathetic nervous system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Systematic proteomic analysis identifies β-site amyloid precursor protein cleaving enzyme 2 and 1 (BACE2 and BACE1) substrates in pancreatic β-cells.The Journal of biological chemistry · 2013Article
- Neuropeptide Y and somatostatin inhibit insulin secretion through different mechanisms.American journal of physiology. Endocrinology and metabolism · 2013Article
- Paracrine and autocrine interactions in the human islet: more than meets the eye.Seminars in cell & developmental biology · 2013Review
- Neuropeptide transmission in brain circuits.Neuron · 2012Review
- Deconstructing pancreas developmental biology.Cold Spring Harbor perspectives in biology · 2012Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
backgroundIn addition to polypeptide hormones, pancreatic endocrine cells synthesize a variety of bioactive molecules including classical transmitters and neuropeptides. While these co-transmitters are thought to play a role in regulating hormone release little is known about how their secretion is regulated. Here I investigate the synthesis and release of neuropeptide Y from pancreatic beta cells. METHODOLOGY/PRINCIPAL
findingsNPY appears to be an authentic co-transmitter in neonatal, but not adult, beta cells because (1) early in mouse post-natal development, many beta cells are NPY-immunoreactive whereas no staining is observed in beta cells from NPY knockout mice; (2) GFP-expressing islet cells from an NPY(GFP) transgenic mouse are insulin-ir; (3) single cell RT-PCR experiments confirm that the NPY(GFP) cells contain insulin mRNA, a marker of beta cells. The NPY-immunoreactivity previously reported in alpha and delta cells is therefore likely to be due to the presence of NPY-related peptides. INS-1 cells, a beta cell line, are also NPY-ir and contain NPY mRNA. Using the FMRFamide tagging technique, NPY secretion was monitored from INS-1 beta cells with high temporal resolution. Peptide release was evoked by brief depolarizations and was potentiated by activators of adenylate cyclase and protein kinase A. Following a transient depolarization, NPY-containing dense core granules fused with the cell membrane and discharged their contents within a few milliseconds.
conclusionsThese results indicate that after birth, NPY expression in pancreatic islets is restricted to neonatal beta cells. The presence of NPY suggests that peptide co-transmitters could mediate rapid paracrine or autocrine signaling within the endocrine pancreas. The FMRFamide tagging technique may be useful in studying the release of other putative islet co-transmitters in real time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.