ArticleThe Journal of biological chemistry2011
Mfc1 is a novel forespore membrane copper transporter in meiotic and sporulating cells.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 42 citations in OpenAlex.
- pka1 deletion induces hyperactivation of the transcription factor Mca1 and drives chromosome mis-segregation in Schizosaccharomyces pombe.Molecular genetics and genomics : MGG · 2026Article
- Induction of postmeiotic DNA double-strand breaks by the Pnu1 endonuclease inMolecular biology of the cell · 2025Article
- A histochemical approach to activity-based copper sensing reveals cuproplasia-dependent vulnerabilities in cancer.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Small-Molecule Fluorescent Probes for Binding- and Activity-Based Sensing of Redox-Active Biological Metals.Chemical reviews · 2024Review
- Article
- Tuning the Color Palette of Fluorescent Copper Sensors through Systematic Heteroatom Substitution at Rhodol Cores.ACS chemical biology · 2018Article
- A role for the transcription factor Mca1 in activating the meiosis-specific copper transporter Mfc1.PloS one · 2018Article
- Cell-surface copper transporters and superoxide dismutase 1 are essential for outgrowth during fungal spore germination.The Journal of biological chemistry · 2017Article
- Analytical Methods for Imaging Metals in Biology: From Transition Metal Metabolism to Transition Metal Signaling.Analytical chemistry · 2017Review
- Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast.G3 (Bethesda, Md.) · 2016Article
- Php4 Is a Key Player for Iron Economy in Meiotic and Sporulating Cells.G3 (Bethesda, Md.) · 2016Article
- Thioether Coordination Chemistry for Molecular Imaging of Copper in Biological Systems.Israel journal of chemistry · 2016Article
- Article
- An antisense RNA-mediated mechanism eliminates a meiosis-specific copper-regulated transcript in mitotic cells.The Journal of biological chemistry · 2015Article
- Recognition- and reactivity-based fluorescent probes for studying transition metal signaling in living systems.Accounts of chemical research · 2015Article
- Synthetic fluorescent probes for studying copper in biological systems.Chemical Society reviews · 2015Review
- Copper is an endogenous modulator of neural circuit spontaneous activity.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- How copper traverses cellular membranes through the mammalian copper transporter 1, Ctr1.Annals of the New York Academy of Sciences · 2014Article
- Characterization of Schizosaccharomyces pombe copper transporter proteins in meiotic and sporulating cells.The Journal of biological chemistry · 2014Article
- Article
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
To gain insight in the molecular basis of copper homeostasis during meiosis, we have used DNA microarrays to analyze meiotic gene expression in the model yeast Schizosaccharomyces pombe. Profiling data identified a novel meiosis-specific gene, termed mfc1(+), that encodes a putative major facilitator superfamily-type transporter. Although Mfc1 does not exhibit any significant sequence homology with the copper permease Ctr4, it contains four putative copper-binding motifs that are typically found in members of the copper transporter family of copper transporters. Similarly to the ctr4(+) gene, the transcription of mfc1(+) was induced by low concentrations of copper. However, its temporal expression profile during meiosis was distinct to ctr4(+). Whereas Ctr4 was observed at the plasma membrane shortly after induction of meiosis, Mfc1 appeared later in precursor vesicles and, subsequently, at the forespore membrane of ascospores. Using the fluorescent copper-binding tracker Coppersensor-1 (CS1), labile cellular copper was primarily detected in the forespores in an mfc1(+)/mfc1(+) strain, whereas an mfc1Δ/mfc1Δ mutant exhibited an intracellular dispersed punctate distribution of labile copper ions. In addition, the copper amine oxidase Cao1, which localized primarily in the forespores of asci, was fully active in mfc1(+)/mfc1(+) cells, but its activity was drastically reduced in an mfc1Δ/mfc1Δ strain. Furthermore, our data showed that meiotic cells that express the mfc1(+) gene have a distinct developmental advantage over mfc1Δ/mfc1Δ mutant cells when copper is limiting. Taken together, the data reveal that Mfc1 serves to transport copper for accurate and timely meiotic differentiation under copper-limiting conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.