ArticleParasites & vectors2011
Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization.
Article in Parasites & vectors, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Biochemistry of Heat Shock Proteins From Human Intracellular Protozoan Parasites as Diagnostic and Therapeutic Biomarkers.Biochemistry · 2025Review
- Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein.Parasites & vectors · 2023Article
- First study on microscopic and molecular detection ofVeterinary world · 2023Article
- Sequencing of hsp70 for discernment of species from the Leishmania (Viannia) guyanensis complex from endemic areas in Colombia.Parasites & vectors · 2022Article
- Molecular identification of two newly identified human pathogens causing leishmaniasis using PCR-based methods on the 3' untranslated region of the heat shock protein 70 (type I) gene.PLoS neglected tropical diseases · 2021Article
- Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis.PLoS neglected tropical diseases · 2020Article
- High resolution melting based method for rapid discriminatory diagnosis of co-infecting Leptomonas seymouri in Leishmania donovani-induced leishmaniasis.Parasitology international · 2020Article
- Complete and de novo assembly of the Leishmania braziliensis (M2904) genome.Memorias do Instituto Oswaldo Cruz · 2018Article
- Real-time PCR applications for diagnosis of leishmaniasis.Parasites & vectors · 2018Review
- Article
- Leishmania braziliensis SCD6 and RBP42 proteins, two factors with RNA binding capacity.Parasites & vectors · 2017Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Molecular Chaperones of Leishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes.BioMed research international · 2015Review
- Expression profiles of the heat shock protein 70 gene in response to heat stress in Agrotis c-nigrum (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2015Article
- Leishmania braziliensis replication protein A subunit 1: molecular modelling, protein expression and analysis of its affinity for both DNA and RNA.Parasites & vectors · 2014Article
- The calmodulin intergenic spacer as molecular target for characterization of Leishmania species.Parasites & vectors · 2014Article
- Alpha tubulin genes from Leishmania braziliensis: genomic organization, gene structure and insights on their expression.BMC genomics · 2013Article
- Sequence analysis of the 3'-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification.Parasites & vectors · 2012Article
- Improving the population genetics toolbox for the study of the African malaria vector Anopheles nili: microsatellite mapping to chromosomes.Parasites & vectors · 2011Article
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe heat stress suffered by Leishmania sp during its digenetic life-cycle is a key trigger for its stage differentiation. In Leishmania subgenera two classes of HSP70 genes differing in their 3' UTR were described. Although the presence of HSP70-I genes was previously suggested in Leishmania (Viannia) braziliensis, HSP70-II genes had been reluctant to be uncovered.
resultsHere, we report the existence of two types of HSP70 genes in L. braziliensis and the genomic organization of the HSP70 locus. RT-PCR experiments were used to map the untranslated regions (UTR) of both types of genes. The 3' UTR-II has a low sequence identity (55-57%) when compared with this region in other Leishmania species. In contrast, the 5' UTR, common to both types of genes, and the 3' UTR-I were found to be highly conserved among all Leishmania species (77-81%). Southern blot assays suggested that L. braziliensis HSP70 gene cluster may contain around 6 tandemly-repeated HSP70-I genes followed by one HSP70-II gene, located at chromosome 28. Northern blot analysis indicated that levels of both types of mRNAs are not affected by heat shock.
conclusionsThis study has led to establishing the composition and structure of the HSP70 locus of L. braziliensis, complementing the information available in the GeneDB genome database for this species. L. braziliensis HSP70 gene regulation does not seem to operate by mRNA stabilization as occurs in other Leishmania species.
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