Evidence map›Paper›PMID 21924172›Full record

ArticleMethods in cell biology2011

Chromatin immunoprecipitation in adult zebrafish red cells.

Eirini Trompouki, Teresa V Bowman, Anthony Dibiase, Yi Zhou, Leonard I Zon

Open access · greenAbstract read
In one paragraph

Article in Methods in cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Bloody Zebrafish: Novel Methods in Normal and Malignant Hematopoiesis.Frontiers in cell and developmental biology · 2018
    Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Eirini TrompoukiStem Cell Program, Children's Hospital Boston, Harvard Stem Cell Institute, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts, USA.
Teresa V Bowman
Anthony Dibiase
Yi Zhou
Leonard I Zon
Howard Hughes Medical Institute · US

Funding

TRANSCRIPTION FACTOR GATA-1 IN TERMINAL ERYTHROID DEVELOPMENTP01HL032262 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI ORKIN, STUART H · 1985 to 2021
$38.9M
EMBRYONIC HEMATOPOIESISR01HL048801 · NHLBI · CHILDREN'S HOSPITAL BOSTON · PI ZON, LEONARD IRA · 1992 to 2016
$5.7M
Identification of Regulators of Hematopoietic Stem Cells Using Zebrafish GeneticsK01DK085270 · NIDDK · BOSTON CHILDREN'S HOSPITAL · PI BOWMAN, TERESA V · 2009 to 2013
$683k
NHLBI NIH HHS 5P01HL32262-29NHLBI NIH HHS 5R01HL048801-18NHLBI NIH HHS P01 HL032262NHLBI NIH HHS R01 HL048801NIDDK NIH HHS K01 DK085270NIDDK NIH HHS K01DK085270-02
6 · The paper itself

Abstract

Zebrafish has been used for many years as a model to study development and disease. The ability of zebrafish to produce thousand of embryos in a synchronous manner has made zebrafish an invaluable tool for genetic and chemical screens. Since its emergence as an important model organism the molecular tools for studying zebrafish have been limited. In this chapter, we describe a simple method to identify DNA binding sites and chromatin architecture in erythrocytes from adult zebrafish using chromatin immunoprecipitation coupled with next generation sequencing. This technique has been used extensively and successfully in other systems and it will be a useful tool for studying epigenetics in zebrafish.

Indexed as

AnimalsChromatinChromatin ImmunoprecipitationCross-Linking ReagentsDNAEpigenesis, GeneticErythrocytesFormaldehydeHigh-Throughput Nucleotide SequencingHistonesProtein BindingSequence Analysis, DNASonicationTissue FixationTranscription FactorsZebrafishChromatinCross-Linking ReagentsDNAFormaldehydeHistonesTranscription Factors

Identifiers

PMID21924172
PMCPMC4712382
OpenAlexW5995806

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.