Evidence mapPaperPMID 21939398Full record

ArticleHuman gene therapy2012

The effects of anti-inflammatory and anti-angiogenic DNA vaccination on diabetic nephropathy in rats.

Peter Celec, Július Hodosy, Roman Gardlík, Michal Behuliak, Roland Pálffy, Marek Pribula, Peter Jáni, Ján Turňa, Katarína Sebeková

Abstract read
In one paragraph

Article in Human gene therapy, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 15 citations in OpenAlex.

  1. Pooled it
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  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Peter CelecInstitute of Molecular Biomedicine, Comenius University , 811 08 Bratislava, Slovakia. petercelec@gmail.com
Július Hodosy
Roman Gardlík
Michal Behuliak
Roland Pálffy
Marek Pribula
Peter Jáni
Ján Turňa
Katarína Sebeková
Comenius University Bratislava · SKHarvard University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Inflammation and angiogenesis play a crucial role in the pathomechanism of diabetic nephropathy. Monocyte chemoattractant protein 1 (MCP) is a key regulator of the immune system in kidneys, and its inhibition with a dominant-negative mutant lacking the N-terminal amino acids 2-8 (7ND) reduces renal fibrosis. Angiomotin (Amot) is a novel angiogenesis modulator. We studied the effects of inhibition of Amot and MCP using DNA vaccination on incipient diabetic nephropathy in rats. Plasmid DNA (with either 7ND or human Amot) was electroporated twice into hind-limb muscles of rats with streptozotocin-induced diabetes mellitus. Sham-electroporated diabetic rats and healthy animals served as controls. After 4 months, renal histology and biochemical analyses were performed. In sham-electroporated diabetic rats, glomerular histology revealed pathological changes. 7ND and Amot treatments reduced glomerular hypertrophy and periodic acid-Schiff positivity. In both treated groups, the expression of profibrotic (transforming growth factor-β, collagen 1), proinflammatory (interleukin-6, tumor necrosis factor-α), and proangiogenic (vascular endothelial growth factor) genes in the renal cortex was lower than in the diabetic group without treatment. The mentioned renoprotective effects could be mediated via higher total antioxidant capacity and improved glycemic control. Anti-angiogenic and anti-inflammatory DNA vaccination ameliorates the progression of glomerular pathology in an animal model of diabetic nephropathy.

Indexed as

AngiomotinsAnimalsChemokine CCL2CollagenDiabetes Mellitus, ExperimentalDiabetic NephropathiesElectroporationFibrosisGene ExpressionHumansIntercellular Signaling Peptides and ProteinsInterleukin-6Kidney GlomerulusMaleMembrane ProteinsNeovascularization, PathologicAmot protein, ratAngiomotinsCcl2 protein, ratChemokine CCL2CollagenIntercellular Signaling Peptides and ProteinsInterleukin-6Membrane ProteinsTransforming Growth Factor betaVaccines, DNAVascular Endothelial Growth Factor Avascular endothelial growth factor A, rat

Identifiers

PMID21939398
PMCPMC3277732
OpenAlexW2008956370

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.