ArticleBiotechnology and bioengineering2012
A novel 3D liver organoid system for elucidation of hepatic glucose metabolism.
Article in Biotechnology and bioengineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Properties of 3-Dimensional Cell Cultivation Matrices and Scaffolds in Magnetic Resonance Imaging at 3 Tesla.Journal of functional biomaterials · 2025Article
- From gut to liver: organoids as platforms for next-generation toxicology assessment vehicles for xenobiotics.Stem cell research & therapy · 2025Review
- Imaging cell spheroid clusters: An MRI protocol for non-invasive standardized characterization.Heliyon · 2025Article
- Organoid-guided precision hepatology for metabolic liver disease.Journal of hepatology · 2024Review
- Organoids transplantation attenuates intestinal ischemia/reperfusion injury in mice through L-Malic acid-mediated M2 macrophage polarization.Nature communications · 2023Article
- Recent advances in organoid engineering: A comprehensive review.Applied materials today · 2022Article
- Measuring Glucose Consumption and Gluconeogenesis in 3D Human Tissue Cultures with Nanoliter Input Volumes.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Advances in removing mass transport limitations for more physiologically relevantBiophysics reviews · 2021Review
- Recent advances in organoid development and applications in disease modeling.Biochimica et biophysica acta. Reviews on cancer · 2021Review
- Liver organoids in domestic animals: an expected promise for metabolic studies.Veterinary research · 2021Review
- Insulin-dependent glucose consumption dynamics in 3D primary human liver cultures measured by a sensitive and specific glucose sensor with nanoliter input volume.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Vibrational Spectroscopy for In Vitro Monitoring Stem Cell Differentiation.Molecules (Basel, Switzerland) · 2020Review
- Development of 3D Hepatic Constructs Within Polysaccharide-Based Scaffolds with Tunable Properties.International journal of molecular sciences · 2020Article
- A liver-specific gene expression panel predicts the differentiation status of in vitro hepatocyte models.Hepatology (Baltimore, Md.) · 2017Article
- Three-dimensional perfused humanWorld journal of gastroenterology · 2017Article
- On the adhesion-cohesion balance and oxygen consumption characteristics of liver organoids.PloS one · 2017Article
- Experimental models of hepatitis B and C - new insights and progress.Nature reviews. Gastroenterology & hepatology · 2016Review
- The hepatocyte proteome in organotypic rat liver models and the influence of the local microenvironment.Proteome science · 2016Article
- Hormone and Drug-Mediated Modulation of Glucose Metabolism in a Microscale Model of the Human Liver.Tissue engineering. Part C, Methods · 2015Article
- Strategies, models and biomarkers in experimental non-alcoholic fatty liver disease research.Progress in lipid research · 2015Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Hepatic glucose metabolism is a key player in diseases such as obesity and diabetes as well as in antihyperglycemic drugs screening. Hepatocytes culture in two-dimensional configurations is limited in vitro model for hepatocytes to function properly, while truly practical platforms to perform three-dimensional (3D) culture are unavailable. In this work, we present a practical organoid culture method of hepatocytes for elucidation of glucose metabolism under nominal and stress conditions. Employing this new method of culturing cells within a hollow fiber reactor, hepatocytes were observed to self-assemble into 3D spherical organoids with preservation of tight junctions and display increased liver-specific functions. Compared to both monolayer culture and sandwich culture, the hepatocyte organoids displayed higher intracellular glycogen content, glucose consumption, and gluconeogenesis and approached the in vivo values, as also confirmed by gene expression of key enzymes. Moreover, hepatocyte organoids demonstrated more realistic sensitivity to hormonal challenges with insulin, glucagon, and dexamethasone. Finally, the exposure to high glucose demonstrated toxicities including alteration of mitochondrial membrane potential, lipid accumulation, and reactive oxygen species formation, similar to the in vivo responses, which was not captured by monolayer cultures. Collectively, hepatocyte organoids mimicked the in vivo functions better than hepatocyte monolayer and sandwich cultures, suggesting suitability for applications such as antihyperglycemic drugs screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.