ArticleMolecular biology of the cell2012
Gap junction assembly: roles for the formation plaque and regulation by the C-terminus of connexin43.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- MicroRNA expression, target genes, and signaling pathways in infants with a ventricular septal defect.Molecular and cellular biochemistry · 2018Trial
- Intercellular Communication via Mitotic Nanotubes is Influenced by Connexin-43 Trafficking and Actin Remodeling.bioRxiv : the preprint server for biology · 2026Article
- Astrocytes Regulate Brain State Transitions.Neurochemical research · 2025Review
- Connexin 43 hemichannels and related diseases.Antibody therapeutics · 2024Review
- Differential Regulation of Hemichannels and Gap Junction Channels by RhoA GTPase and Actin Cytoskeleton: A Comparative Analysis of Cx43 and Cx26.International journal of molecular sciences · 2024Article
- Review
- Connexins in the Heart: Regulation, Function and Involvement in Cardiac Disease.International journal of molecular sciences · 2021Review
- NLR-1/CASPR Anchors F-Actin to Promote Gap Junction Formation.Developmental cell · 2020Article
- Facets of Communication: Gap Junction Ultrastructure and Function in Cancer Stem Cells and Tumor Cells.Cancers · 2019Review
- Dynamic intercellular transport modulates the spatial patterning of differentiation during early neural commitment.Nature communications · 2018Article
- Spatio-temporal regulation of connexin43 phosphorylation and gap junction dynamics.Biochimica et biophysica acta. Biomembranes · 2018Review
- Freeze fracture: new avenues for the ultrastructural analysis of cells in vitro.Histochemistry and cell biology · 2018Review
- Electrical synapses in mammalian CNS: Past eras, present focus and future directions.Biochimica et biophysica acta. Biomembranes · 2018Review
- Localisation Microscopy of Breast Epithelial ErbB-2 Receptors and Gap Junctions: Trafficking after γ-Irradiation, Neuregulin-1β, and Trastuzumab Application.International journal of molecular sciences · 2017Article
- Connexin Hemichannels: Methods for Dye Uptake and Leakage.The Journal of membrane biology · 2016Article
- Ultrastructural demonstration of Cx43 gap junctions in induced pluripotent stem cells from human cord blood.Histochemistry and cell biology · 2016Article
- Molecular mechanisms regulating formation, trafficking and processing of annular gap junctions.BMC cell biology · 2016Review
- KV1 channels identified in rodent myelinated axons, linked to Cx29 in innermost myelin: support for electrically active myelin in mammalian saltatory conduction.Journal of neurophysiology · 2016Article
- The molecular and immunochemical expression of innexins in the yellow fever mosquito, Aedes aegypti: insights into putative life stage- and tissue-specific functions of gap junctions.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · 2015Article
- Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Using an established gap junction (GJ) assembly system with experimentally reaggregated cells, we analyzed "formation plaques" (FPs), apparent sites of GJ assembly. Employing freeze-fracture electron microscopy methods combined with filipin labeling of sterols and immunolabeling for connexin43 (Cx43), we demonstrated that FPs constitute distinct membrane "domains" and that their characteristic 10-nm particles contain connexin43, thus representing precursors (i.e., GJ hemichannels) engaged in assembly. Analysis of FPs in new systems-HeLa and N2A cells-resolved questions surrounding several key but poorly understood steps in assembly, including matching of FP membranes in apposed cells, reduction in the separation between FP membranes during assembly, and the process of particle aggregation. Findings also indicated that "docking" of GJ hemichannels occurs within FP domains and contributes to reduction of intermembrane separation between FPs. Other experiments demonstrated that FPs develop following a major C-terminal truncation of Cx43 (M257), although assembly was delayed. Particle aggregation also occurred at lower densities, and densities of particles within developing GJ aggregates failed to achieve full-length levels. With regard to regulation, inhibition of assembly following protein kinase C activation failed to occur in the M257 truncation mutants, as measured by intercellular dye transfer. However, several C-terminal serine mutations failed to disrupt inhibition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.