ArticleGenetics2012
MicroRNA transgene overexpression complements deficiency-based modifier screens in Drosophila.
Article in Genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Resources and Methods for the Analysis of MicroRNA Function in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2022Review
- The Application of Extracellular Vesicles Mediated miRNAs in Osteoarthritis: Current Knowledge and Perspective.Journal of inflammation research · 2022Review
- A comprehensive in vivo screen for anti-apoptotic miRNAs indicates broad capacities for oncogenic synergy.Developmental biology · 2021Article
- The in vivo genetic toolkit for studying expression and functions of Drosophila melanogaster microRNAs.RNA biology · 2017Review
- Evolutionary Transitions of MicroRNA-Target Pairs.Genome biology and evolution · 2016Article
- An accelerated miRNA-based screen implicates Atf-3 in Drosophila odorant receptor expression.Scientific reports · 2016Article
- Glial cell regulation of rhythmic behavior.Methods in enzymology · 2015Review
- Genome-wide screen for modifiers of Na (+) /K (+) ATPase alleles identifies critical genetic loci.Molecular brain · 2014Article
- A rapid genome-wide microRNA screen identifies miR-14 as a modulator of Hedgehog signaling.Cell reports · 2014Article
- Review
- Maternal loss of miRNAs leads to increased variance in primordial germ cell numbers in Drosophila melanogaster.G3 (Bethesda, Md.) · 2013Article
- The Hippo pathway acts via p53 and microRNAs to control proliferation and proapoptotic gene expression during tissue growth.Biology open · 2013Article
- Adult-specific functions of animal microRNAs.Nature reviews. Genetics · 2013Review
- Genetic modifier screens to identify components of a redox-regulated cell adhesion and migration pathway.Methods in enzymology · 2013Article
- Article
- Article
- MicroRNAs shape the neuronal landscape.Neuron · 2012Review
- A genome-wide transgenic resource for conditional expression of Drosophila microRNAs.Development (Cambridge, England) · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dosage-sensitive modifier screening is a powerful tool for linking genes to biological processes. Use of chromosomal deletions permits sampling the effects of removing groups of genes related by position on the chromosome. Here, we explore the use of inducible microRNA transgenes as a complement to deficiency-based modifier screens. miRNAs are predicted to have hundreds of targets. miRNA overexpression provides an efficient means to reduces expression of large gene sets. A collection of transgenes was prepared to allow overexpression of 89 miRNAs or miRNA clusters. These transgenes and a set of genomic deficiencies were screened for their ability to modify the bristle phenotype of the cell-cycle regulator minus. Sixteen miRNAs were identified as dominant suppressors, while the deficiency screen uncovered four genomic regions that contain a dominant suppressor. Comparing the genes uncovered by the deletions with predicted miRNA targets uncovered a small set of candidate suppressors. Two candidates were identified as suppressors of the minus phenotype, Cullin-4 and CG5199/Cut8. Additionally, we show that Cullin-4 acts through its substrate receptor Cdt2 to suppress the minus phenotype. We suggest that inducible microRNA transgenes are a useful complement to deficiency-based modifier screens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.