ArticleEukaryotic cell2012
Dynamic localization of Trypanosoma brucei mitochondrial DNA polymerase ID.
Article in Eukaryotic cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- A genome-wide genetic screen identifies a novel kDNA replication protein in trypanosomes.Nucleic acids research · 2026Article
- Translesion synthesis and microhomology-mediated end-joining repair in trypanosomatids.PLoS neglected tropical diseases · 2025Review
- An independently tunable dual control system for RNAi complementation in Trypanosoma brucei.PloS one · 2025Article
- Mitochondrial genome maintenance-the kinetoplast story.FEMS microbiology reviews · 2023Review
- Characterization of two novel proteins involved in mitochondrial DNA anchoring in Trypanosoma brucei.PLoS pathogens · 2023Article
- Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei.Nature microbiology · 2023Article
- Article
- A comparison of three approaches for the discovery of novel tripartite attachment complex proteins in Trypanosoma brucei.PLoS neglected tropical diseases · 2020Article
- A DNA polymerization-independent role for mitochondrial DNA polymerase I-like protein C in African trypanosomes.Journal of cell science · 2020Article
- Cell cycle localization dynamics of mitochondrial DNA polymerase IC in African trypanosomes.Molecular biology of the cell · 2018Article
- A leucine aminopeptidase is involved in kinetoplast DNA segregation in Trypanosoma brucei.PLoS pathogens · 2017Article
- U-insertion/deletion RNA editing multiprotein complexes and mitochondrial ribosomes in Leishmania tarentolae are located in antipodal nodes adjacent to the kinetoplast DNA.Mitochondrion · 2015Article
- Article
- A passion for parasites.The Journal of biological chemistry · 2014Article
- Dual functions of α-ketoglutarate dehydrogenase E2 in the Krebs cycle and mitochondrial DNA inheritance in Trypanosoma brucei.Eukaryotic cell · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Trypanosomes contain a unique form of mitochondrial DNA called kinetoplast DNA (kDNA) that is a catenated network composed of minicircles and maxicircles. Several proteins are essential for network replication, and most of these localize to the antipodal sites or the kinetoflagellar zone. Essential components for kDNA synthesis include three mitochondrial DNA polymerases TbPOLIB, TbPOLIC, and TbPOLID). In contrast to other kDNA replication proteins, TbPOLID was previously reported to localize throughout the mitochondrial matrix. This spatial distribution suggests that TbPOLID requires redistribution to engage in kDNA replication. Here, we characterize the subcellular distribution of TbPOLID with respect to the Trypanosoma brucei cell cycle using immunofluorescence microscopy. Our analyses demonstrate that in addition to the previously reported matrix localization, TbPOLID was detected as discrete foci near the kDNA. TbPOLID foci colocalized with replicating minicircles at antipodal sites in a specific subset of the cells during stages II and III of kDNA replication. Additionally, the TbPOLID foci were stable following the inhibition of protein synthesis, detergent extraction, and DNase treatment. Taken together, these data demonstrate that TbPOLID has a dynamic localization that allows it to be spatially and temporally available to perform its role in kDNA replication.
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