ArticlePlant cell reports2012
Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.).
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 58 citations in OpenAlex.
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- Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Biology · 2022Article
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- Harnessing tissue-specific genome editing in plants through CRISPR/Cas system: current state and future prospects.Planta · 2021Review
- A reporter for noninvasively monitoring gene expression and plant transformation.Horticulture research · 2020Article
- A key variant in the cis-regulatory element of flowering gene Ghd8 associated with cold tolerance in rice.Scientific reports · 2019Article
- Gene Expression Profiling of the Shoot Meristematic Tissues in Woodland StrawberryFrontiers in plant science · 2019Article
- Isolation and Characterization of a Green-Tissue Promoter from Common Wild Rice (International journal of molecular sciences · 2018Article
- Isolation of five rice nonendosperm tissue-expressed promoters and evaluation of their activities in transgenic rice.Plant biotechnology journal · 2018Article
- Switchgrass (Biotechnology for biofuels · 2018Article
- Application of Cre-lox gene switch to limit the Cry expression in rice green tissues.Scientific reports · 2017Article
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- Isolation and Functional Characterization of Bidirectional Promoters in Rice.Frontiers in plant science · 2016Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledIn plant genetic engineering, using tissue-specific promoters to control the expression of target gene is an effective way to avoid potential negative effects of using constitutive promoter, such as metabolic burden and so on. However, until now, there are few tissue-specific promoters with strong and reliable expression that could be used in crop biotechnology application. In this study, based on microarray and RT-PCR data, we identified a rice green tissue-specific expression gene DX1 (LOC_Os12g33120). The expression pattern of DX1 gene promoter was examined by using the β-glucuronidase (GUS) reporter gene and analyzed in transgenic rice plants in different tissues. Histochemical assays and quantitative analyses of GUS activity confirmed that P (DX1):GUS was highly expressed in green tissues. To identify the regulatory elements controlling the expression of the DX1 gene, a series of 5' and 3' deletions of DX1 promoter were fused to GUS gene and stably introduced into rice plants. In addition, gel mobility shift assays and site-directed mutagenesis studies were used, allowing for the identification of two novel tissue-specific cis-acting elements (GSE1 and GSE2) within P(DX1). GSE1 acted as a positive regulator in all green tissues (leaf, sheath, stem and panicle). Compared with GSE1, GSE2 acted as a positive regulator only in sheath and stem tissue, and had a weaker effect on gene expression. In addition, P(DX1):GUS was not expressed in anther and seed, this characteristic reduced the potential ecological risk and potential food safety issues. Taken together, our results strongly suggest that the identified promoter, P(DX1), and its cis regulatory elements, GSE1 and GSE2, are potentially useful in the field of rice transgenic breeding. KEY MESSAGE: We have isolated and characterized the rice green tissue-specific promoter P(DX1), and identified two novel positive cis-acting elements in P(DX1).
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