Evidence mapPaperPMID 22406532Full record

Trial reportThe Journal of clinical investigation2012

Niacin and biosynthesis of PGD₂by platelet COX-1 in mice and humans.

Wen-Liang Song, Jane Stubbe, Emanuela Ricciotti, Naji Alamuddin, Salam Ibrahim, Irene Crichton, Maxwell Prempeh, John A Lawson, Robert L Wilensky, Lars Melholt Rasmussen and 2 more

Registry-linked trialOpen access · greenAbstract readComparative StudyRandomized Controlled Trial
In one paragraph

Trial report in The Journal of clinical investigation, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It reports registered trial NCT01275300. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.8field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01275300 nacompleted

A Pilot Study to Measure Plasma and Urinary Prostaglandin D2 Metabolites Evoked by Niacin

Ran2007Enrolled9Registered outcomes1Posted comparisons0ConditionsHyperlipidemiaArmsAspirin, niacin, Placebo
Open the trial in the graph
3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 67 citations in OpenAlex.

  1. Inherited human group IVA cytosolic phospholipase A2 deficiency abolishes platelet, endothelial, and leucocyte eicosanoid generation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2015
    Trial
  2. Trial
  3. Review
  4. Prostanoids in Cardiac and Vascular Remodeling.Arteriosclerosis, thrombosis, and vascular biology · 2024
    Review
  5. Article
  6. Article
  7. Article
  8. Regulatory Roles of Phospholipase AFrontiers in immunology · 2022
    Review
  9. Article
  10. Profiling the eicosanoid networks that underlie the anti- and pro-thrombotic effects of aspirin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
  11. Article
  12. Article
  13. Review
  14. The expansive role of oxylipins on platelet biology.Journal of molecular medicine (Berlin, Germany) · 2017
    Review
  15. Article
  16. Review
  17. Review
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Wen-Liang SongInstitute for Translational Medicine and Therapeutics, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania 19104, USA.
Jane Stubbe
Emanuela Ricciotti
Naji Alamuddin
Salam Ibrahim
Irene Crichton
Maxwell Prempeh
John A Lawson
Robert L Wilensky
Lars Melholt Rasmussen
Ellen Puré
Garret A FitzGerald
Translational Therapeutics (United States) · USThe Wistar Institute · USUniversity of Pennsylvania · US

Funding

STUDIES OF PLATELET ADHERENCE TO OSTEOPONTINP01HL062250 · UNIVERSITY OF PENNSYLVANIA · 1999 to 2005
$10.0M
NCRR NIH HHS UL1 RR024134NCRR NIH HHS UL1-RR-024134NHLBI NIH HHS HL-62250NHLBI NIH HHS HL-83799NHLBI NIH HHS P01 HL062250NHLBI NIH HHS P50 HL083799
6 · The paper itself

Abstract

The clinical use of niacin to treat dyslipidemic conditions is limited by noxious side effects, most commonly facial flushing. In mice, niacin-induced flushing results from COX-1-dependent formation of PGD₂ and PGE₂ followed by COX-2-dependent production of PGE₂. Consistent with this, niacin-induced flushing in humans is attenuated when niacin is combined with an antagonist of the PGD₂ receptor DP1. NSAID-mediated suppression of COX-2-derived PGI₂ has negative cardiovascular consequences, yet little is known about the cardiovascular biology of PGD₂. Here, we show that PGD₂ biosynthesis is augmented during platelet activation in humans and, although vascular expression of DP1 is conserved between humans and mice, platelet DP1 is not present in mice. Despite this, DP1 deletion in mice augmented aneurysm formation and the hypertensive response to Ang II and accelerated atherogenesis and thrombogenesis. Furthermore, COX inhibitors in humans, as well as platelet depletion, COX-1 knockdown, and COX-2 deletion in mice, revealed that niacin evoked platelet COX-1-derived PGD₂ biosynthesis. Finally, ADP-induced spreading on fibrinogen was augmented by niacin in washed human platelets, coincident with increased thromboxane (Tx) formation. However, in platelet-rich plasma, where formation of both Tx and PGD₂ was increased, spreading was not as pronounced and was inhibited by DP1 activation. Thus, PGD₂, like PGI₂, may function as a homeostatic response to thrombogenic and hypertensive stimuli and may have particular relevance as a constraint on platelets during niacin therapy.

Indexed as

6-Ketoprostaglandin F1 alphaAdenosine DiphosphateAngioplasty, Balloon, CoronaryAnimalsAortic Aneurysm, AbdominalApolipoproteins EAtherosclerosisBlood PlateletsCarotid Artery ThrombosisCyclooxygenase 1Cyclooxygenase 2Cyclooxygenase InhibitorsDouble-Blind MethodEndothelium, VascularFemaleHumans2,3-dinor-6-ketoprostaglandin F1alpha6-Ketoprostaglandin F1 alphaAdenosine DiphosphateApolipoproteins ECyclooxygenase 1Cyclooxygenase 2Cyclooxygenase InhibitorsHCAR2 protein, humanMembrane ProteinsPlatelet Aggregation InhibitorsProstaglandin D2prostaglandin D2 receptorprostanoid D receptor 1, mousePTGS1 protein, humanPtgs1 protein, mousePtgs2 protein, mouseReceptors, G-Protein-CoupledReceptors, ImmunologicReceptors, LDLReceptors, NicotinicReceptors, ProstaglandinThromboxane A2

Identifiers

PMID22406532
PMCPMC3314457
OpenAlexW2034845019

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.