ArticleThe Journal of biological chemistry2012
A 43-nucleotide U-rich element in 3'-untranslated region of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotes.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Variation in surface protein expression leads to heterogeneous Trypanosoma cruzi populations during host cell infection.Nature communications · 2025Article
- Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation.Cancer cell international · 2025Article
- Transcriptomic analysis of N-terminal mutated Trypanosoma cruzi UBP1 knockdown underlines the importance of this RNA-binding protein in parasite development.PLoS neglected tropical diseases · 2024Article
- RNA-seq data exploration after trypanosome RNA-binding protein UBP1 expression is altered by CRISPR-Cas9 gene editing and overexpression.Data in brief · 2024Article
- Signaling pathways involved in environmental sensing in Trypanosoma cruzi.Molecular microbiology · 2021Review
- Article
- Membrane-bound extracellular vesicles secreted by parasitic protozoa: cellular structures involved in the communication between cells.Parasitology research · 2020Review
- RNA Binding Proteins and Gene Expression Regulation inFrontiers in cellular and infection microbiology · 2020Article
- The RNA-binding protein TcUBP1 up-regulates an RNA regulon for a cell surface-associatedThe Journal of biological chemistry · 2019Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Nuclear Compartmentalization Contributes to Stage-Specific Gene Expression Control inFrontiers in cell and developmental biology · 2017Article
- Transcriptome Remodeling in Trypanosoma cruzi and Human Cells during Intracellular Infection.PLoS pathogens · 2016Article
- Purification of Messenger Ribonucleoprotein Particles via a Tagged Nascent Polypeptide.PloS one · 2016Article
- Genome evolution in trypanosomatid parasites.Parasitology · 2015Review
- Distinct genomic organization, mRNA expression and cellular localization of members of two amastin sub-families present in Trypanosoma cruzi.BMC microbiology · 2013Article
- Article
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
Trypanosoma cruzi, the agent of Chagas disease, does not seem to control gene expression through regulation of transcription initiation and makes use of post-transcriptional mechanisms. We report here a 43-nt U-rich RNA element located in the 3'-untranslated region (3'-UTR) of a large number of T. cruzi mRNAs that is important for mRNA abundance in the intracellular amastigote stage of the parasite. Whole genome scan analysis, differential display RT-PCR, Northern blot, and RT-PCR analyses were used to determine the transcript levels of more than 900 U-rich-containing mRNAs of large gene families as well as single and low copy number genes. Our results indicate that the 43-nt U-rich mRNA element is preferentially present in amastigotes. The cis-element of a protein kinase 3'-UTR but not its mutated version promoted the expression of the green fluorescent protein reporter gene in amastigotes. The regulatory cis-element, but not its mutated version, was also shown to interact with the trypanosome-specific RNA-binding protein (RBP) TcUBP1 but not with other related RBPs. Co-immunoprecipitation experiments of TcUBP1-containing ribonucleoprotein complexes formed in vivo validated the interaction with representative endogenous RNAs having the element. These results suggest that this 43-nt U-rich element together with other yet unidentified sequences might be involved in the modulation of abundance and/or translation of subsets of transcripts in the amastigote stage.
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