ArticleMolecular & cellular proteomics : MCP2012
DNA ends alter the molecular composition and localization of Ku multicomponent complexes.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Ku limits aberrant mRNA splicing promoted by intronic antisense Alu elements.bioRxiv : the preprint server for biology · 2025Article
- The knock-in atlas: a web resource for targeted protein trap by CRISPR/Cas9 in human and mouse cell lines.Nucleic acids research · 2025Article
- DEPDC5 regulates the strength of excitatory synaptic transmission by interacting with ubiquitin-specific protease 46.Neurobiology of disease · 2025Article
- Review
- The Ku complex: recent advances and emerging roles outside of non-homologous end-joining.Cellular and molecular life sciences : CMLS · 2021Review
- TRIP13 regulates DNA repair pathway choice through REV7 conformational change.Nature cell biology · 2020Article
- Tandem Affinity Purification and Mass Spectrometry (TAP-MS) for the Analysis of Protein Complexes.Current protocols in protein science · 2019Article
- A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification.Journal of the American Chemical Society · 2019Article
- Stabilization of the methyl-CpG binding protein ZBTB38 by the deubiquitinase USP9X limits the occurrence and toxicity of oxidative stress in human cells.Nucleic acids research · 2018Article
- STK40 Is a Pseudokinase that Binds the E3 Ubiquitin Ligase COP1.Structure (London, England : 1993) · 2017Article
- Structural Basis for Substrate Selectivity of the E3 Ligase COP1.Structure (London, England : 1993) · 2016Article
- Peptidic degron in EID1 is recognized by an SCF E3 ligase complex containing the orphan F-box protein FBXO21.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mutations in G protein β subunits promote transformation and kinase inhibitor resistance.Nature medicine · 2015Article
- Proteomic analysis reveals CACN-1 is a component of the spliceosome in Caenorhabditis elegans.G3 (Bethesda, Md.) · 2014Article
- An amino terminal phosphorylation motif regulates intranuclear compartmentalization of Olig2 in neural progenitor cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- SOX2 and p63 colocalize at genetic loci in squamous cell carcinomas.The Journal of clinical investigation · 2014Article
- Emerging roles of the nucleolus in regulating the DNA damage response: the noncanonical DNA repair enzyme APE1/Ref-1 as a paradigmatical example.Antioxidants & redox signaling · 2014Review
- SIRT1 gene expression upon genotoxic damage is regulated by APE1 through nCaRE-promoter elements.Molecular biology of the cell · 2014Article
- Article
- RNA recognition by the DNA end-binding Ku heterodimer.RNA (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 1 country.
Funding
Abstract
The Ku heterodimer plays an essential role in non-homologous end-joining and other cellular processes including transcription, telomere maintenance and apoptosis. While the function of Ku is regulated through its association with other proteins and nucleic acids, the specific composition of these macromolecular complexes and their dynamic response to endogenous and exogenous cellular stimuli are not well understood. Here we use quantitative proteomics to define the composition of Ku multicomponent complexes and demonstrate that they are dramatically altered in response to UV radiation. Subsequent biochemical assays revealed that the presence of DNA ends leads to the substitution of RNA-binding proteins with DNA and chromatin associated factors to create a macromolecular complex poised for DNA repair. We observed that dynamic remodeling of the Ku complex coincided with exit of Ku and other DNA repair proteins from the nucleolus. Microinjection of sheared DNA into live cells as a mimetic for double strand breaks confirmed these findings in vivo.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.