ArticleParasites & vectors2012
Sequence analysis of the 3'-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification.
Article in Parasites & vectors, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 38 citations in OpenAlex.
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- Prokaryotic and eukaryotic skin microbiota modifications triggered by Leishmania infection in localized Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2024Article
- Cutaneous and Visceral Leishmaniasis Caused by the Same Zymodeme of Leishmania donovani in Kerala, India.The American journal of tropical medicine and hygiene · 2024Article
- Novel duplex TaqMan-based quantitative PCR for rapid and accurate diagnosis ofCurrent research in parasitology & vector-borne diseases · 2024Article
- Natural infection withFrontiers in microbiology · 2023Article
- Molecular detection of Leishmania infantum in rats and sand flies in the urban sewers of Barcelona, Spain.Parasites & vectors · 2022Article
- Molecular identification of two newly identified human pathogens causing leishmaniasis using PCR-based methods on the 3' untranslated region of the heat shock protein 70 (type I) gene.PLoS neglected tropical diseases · 2021Article
- Dipeptidyl peptidase III as a DNA marker to investigate epidemiology and taxonomy of Old World Leishmania species.PLoS neglected tropical diseases · 2021Article
- Advances in UnderstandingFrontiers in cell and developmental biology · 2021Review
- Distribution of Phlebotomine Sandflies in the Cave Area of Satun Province, Thailand.Tropical medicine and infectious disease · 2020Article
- Untranslated regions of mRNA and their role in regulation of gene expression in protozoan parasites.Journal of biosciences · 2017Review
- Species typing in dermal leishmaniasis.Clinical microbiology reviews · 2015Review
- The wide expansion of hepatitis delta virus-like ribozymes throughout trypanosomatid genomes is linked to the spreading of L1Tc/ingi clade mobile elements.BMC genomics · 2014Article
- The calmodulin intergenic spacer as molecular target for characterization of Leishmania species.Parasites & vectors · 2014Article
- Multilocus microsatellite signature and identification of specific molecular markers for Leishmania aethiopica.Parasites & vectors · 2013Article
- Species Identification and Molecular Typing ofIranian journal of parasitologyArticle
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Leishmaniases are a group of clinically diverse diseases caused by parasites of the genus Leishmania. To distinguish between species is crucial for correct diagnosis and prognosis as well as for treatment decisions. Recently, sequencing of the HSP70 coding region has been applied in phylogenetic studies and for identifying of Leishmania species with excellent results.
methodsIn the present study, we analyzed the 3'-untranslated region (UTR) of Leishmania HSP70-type I gene from 24 strains representing eleven Leishmania species in the belief that this non-coding region would have a better discriminatory capacity for species typing than coding regions.
resultsIt was observed that there was a remarkable degree of sequence conservation in this region, even between species of the subgenus Leishmania and Viannia. In addition, the presence of many microsatellites was a common feature of the 3'-UTR of HSP70-I genes in the Leishmania genus. Finally, we constructed dendrograms based on global sequence alignments of the analyzed Leishmania species and strains, the results indicated that this particular region of HSP70 genes might be useful for species (or species complex) typing, improving for particular species the discrimination capacity of phylogenetic trees based on HSP70 coding sequences. Given the large size variation of the analyzed region between the Leishmania and Viannia subgenera, direct visualization of the PCR amplification product would allow discrimination between subgenera, and a HaeIII-PCR-RFLP analysis might be used for differentiating some species within each subgenera.
conclusionsSequence and phylogenetic analyses indicated that this region, which is readily amplified using a single pair of primers from both Old and New World Leishmania species, might be useful as a molecular marker for species discrimination.
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