ArticleProceedings of the National Academy of Sciences of the United States of America1990
Glucose-induced translocation of protein kinase C in rat pancreatic islets.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Who cites it
28 citing papers in PubMed, 95 citations in OpenAlex.
- Antox targeting AGE/RAGE cascades to restore submandibular gland viability in rat model of type 1 diabetes.Scientific reports · 2024Article
- Vascular endothelial growth factor B inhibits insulin secretion in MIN6 cells and reduces CaWorld journal of diabetes · 2021Article
- Reduced Expression ofFrontiers in endocrinology · 2019Article
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Expression of Protein Kinase C Isoforms in Pancreatic Islets and Liver of Male Goto-Kakizaki Rats, a Model of Type 2 Diabetes.PloS one · 2015Article
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- Biphasic insulin secretion from freshly isolated or cultured, perifused rodent islets: comparative studies with rats and mice.Metabolism: clinical and experimental · 2008Article
- Bimodal role of conventional protein kinase C in insulin secretion from rat pancreatic beta cells.The Journal of physiology · 2004Article
- Fatty acid metabolism and insulin secretion in pancreatic beta cells.Diabetologia · 2003Review
- Phosphorylation of SNAP-25 on serine-187 is induced by secretagogues in insulin-secreting cells, but is not correlated with insulin secretion.The Biochemical journal · 2002Article
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- Stimulation of fructose transport across the intestinal brush-border membrane by PMA is mediated by GLUT2 and dynamically regulated by protein kinase C.The Biochemical journal · 2000Article
- The diffusive component of intestinal glucose absorption is mediated by the glucose-induced recruitment of GLUT2 to the brush-border membrane.The Biochemical journal · 2000Article
- Analysis of the PKC-gamma-related immunocrossreactive region of a novel leukocyte protein gamma-rp.Inflammation · 1999Article
- Aspects of novel sites of regulation of the insulin stimulus-secretion coupling in normal and diabetic pancreatic islets.Endocrine · 1998Review
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- Diversity of roles of protein kinase C alpha in the proliferation of Swiss 3T3 cells.The Biochemical journal · 1996Article
- Dissociation between changes in cytoplasmic free Ca2+ concentration and insulin secretion as evidenced from measurements in mouse single pancreatic islets.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Effects of short-term culturing on islet phosphoinositide and insulin secretory responses to glucose and carbachol.Acta diabetologica · 1995Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The role of protein kinase C (PKC) as a mediator of glucose-induced insulin secretion has been a subject of controversy. Glucose-induced translocation of PKC has not been reported, and the relevant PKC isoenzymes in islets have not been identified. To address these issues, we developed specific antibodies to the alpha, beta, and gamma isoenzymes of PKC. Western blots of homogenates of freshly isolated rat islets probed with these antibodies revealed that the major isoenzyme present is alpha-PKC. Islets were perifused for 15 min with either 2.75 mM glucose, 20 mM glucose, 20 mM glucose plus 30 mM mannoheptulose, 15 mM alpha-ketoisocaproate, or alpha-ketoisocaproate plus mannoheptulose. Quantitative immunoblotting of membrane and cytosol fractions showed that alpha-PKC translocated from the cytosol to the membrane in freshly isolated rat islets stimulated with either 20 mM glucose or 15 mM alpha-ketoisocaproate. Both the secretory response and the translocation of alpha-PKC were blocked by the addition of mannoheptulose, an inhibitor of glucose metabolism, in islets stimulated with glucose but not in islets stimulated with alpha-ketoisocaproate. These results support a role for alpha-PKC in mediating glucose-induced insulin secretion in pancreatic islets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.