ArticleMicrobiology (Reading, England)2012
Vibrio parahaemolyticus ExsE is requisite for initial adhesion and subsequent type III secretion system 1-dependent autophagy in HeLa cells.
Article in Microbiology (Reading, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
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- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- Sublethal Curcumin Exposure Induces Global Gene Expression and Biofilm-Related Phenotypic Changes in Vibrio parahaemolyticus.Current microbiology · 2025Article
- Transcriptomic Profiles of Vibrio parahaemolyticus During Biofilm Formation.Current microbiology · 2023Article
- A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility inPathogens (Basel, Switzerland) · 2022Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- Type III Secretion 1 Effector Gene Diversity AmongFrontiers in cellular and infection microbiology · 2020Article
- Fitting Pieces into the Puzzle ofJournal of bacteriology · 2019Review
- Identification of a Novel Small RNAFrontiers in microbiology · 2018Article
- Autoregulation of ToxR and Its Regulatory Actions on Major Virulence Gene Loci inFrontiers in cellular and infection microbiology · 2018Article
- Regulatory actions of ToxR and CalR on their own genes and type III secretion system 1 inOncotarget · 2017Article
- ExsE Is a Negative Regulator for T3SS Gene Expression inFrontiers in cellular and infection microbiology · 2016Article
- Transcriptome analysis of Vibrio parahaemolyticus in type III secretion system 1 inducing conditions.Frontiers in cellular and infection microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio parahaemolyticus pandemic serotype O3 : K6 causes acute gastroenteritis, wound infections and septicaemia in humans. This organism encodes two type III secretion systems (T3SS1 and T3SS2); host-cell cytotoxicity has been attributed to T3SS1. Synthesis and secretion of T3SS1 proteins is positively regulated by ExsA, which is presumptively regulated by the ExsCDE pathway, similar to Pseudomonas aeruginosa. Herein we deleted the putative exsE from V. parahaemolyticus and found constitutive expression of the T3SS1 in broth culture as expected. More importantly, however, in a cell culture model, the ΔexsE strain was unable to induce cytotoxicity, as measured by release of lactate dehydrogenase (LDH), or autophagy, as measured by LC3 conversion. This is markedly different from P. aeruginosa, where deletion of exsE has no effect on host-cell cytolysis. Swarming and cytoadhesion were reduced for the deletion mutant and could be recovered along with T3SS1-induced HeLa cell cytotoxicity by in cis expression of exsE in the ΔexsE strain. Loss of adhesion and swarming motility was associated with the loss of flagella biogenesis in the exsE-deficient strain. Mouse mortality was unaffected by the deletion of exsE compared with a wild-type control, suggesting that additional adhesins are important for intoxication in vivo. Based on these data, we conclude that ExsE contributes to the negative regulation of T3SS1 and, in addition, contributes to regulation of an adherence phenotype that is requisite for translocation of effector proteins into HeLa cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.