Evidence map›Paper›PMID 22854634›Full record

ArticleIntegrative biology : quantitative biosciences from nano to macro2012

Soft microenvironments promote the early neurogenic differentiation but not self-renewal of human pluripotent stem cells.

Albert J Keung, Prashanth Asuri, Sanjay Kumar, David V Schaffer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Integrative biology : quantitative biosciences from nano to macro, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed.

  1. Review
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  6. Article
  7. Toward Bioactive Hydrogels: A Tunable Approach via Nucleic Acid-Collagen Complexation.Regenerative engineering and translational medicine · 2024
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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Albert J KeungDepartment of Chemical and Biomolecular Engineering, University of California, Berkeley, CA 94720, USA.
Prashanth Asuri
Sanjay Kumar
David V Schaffer

Funding

Cellular mechanobiology: Biophysics and therapeuticsDP2OD004213 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI KUMAR, SANJAY · 2008 to 2008
$2.3M
NIH HHS 1DP2OD004213NIH HHS DP2 OD004213
6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) are of great interest in biology and medicine due to their ability to self-renew and differentiate into any adult or fetal cell type. Important efforts have identified biochemical factors, signaling pathways, and transcriptional networks that regulate hPSC biology. However, recent work investigating the effect of biophysical cues on mammalian cells and adult stem cells suggests that the mechanical properties of the microenvironment, such as stiffness, may also regulate hPSC behavior. While several studies have explored this mechanoregulation in mouse embryonic stem cells (mESCs), it has been challenging to extrapolate these findings and thereby explore their biomedical implications in hPSCs. For example, it remains unclear whether hPSCs can be driven down a given tissue lineage by providing tissue-mimetic stiffness cues. Here we address this open question by investigating the regulation of hPSC neurogenesis by microenvironmental stiffness. We find that increasing extracellular matrix (ECM) stiffness in vitro increases hPSC cell and colony spread area but does not alter self-renewal, in contrast to past studies with mESCs. However, softer ECMs with stiffnesses similar to that of neural tissue promote the generation of early neural ectoderm. This mechanosensitive increase in neural ectoderm requires only a short 5-day soft stiffness "pulse", which translates into downstream increases in both total neurons as well as therapeutically relevant dopaminergic neurons. These findings further highlight important differences between mESCs and hPSCs and have implications for both the design of future biomaterials as well as our understanding of early embryonic development.

Indexed as

Cell Culture TechniquesCell DifferentiationDopaminergic NeuronsExtracellular MatrixFlow CytometryHumansMicroscopy, Atomic ForceMicroscopy, Phase-ContrastNeurogenesisPluripotent Stem CellsStress, Mechanical

Identifiers

PMID22854634
PMCPMC3459311

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.