ArticleBMC musculoskeletal disorders2012
Collagen type I and decorin expression in tenocytes depend on the cell isolation method.
Article in BMC musculoskeletal disorders, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Sequential vascular and neural changes underlying the progression of patellar tendinopathy.Journal of orthopaedic surgery and research · 2026Article
- An in vitro collagen gel contraction assay to assess the relaxing effect of potential pharmacological alternatives to oxytetracycline on foals' tendons.Scientific reports · 2026Article
- Comparative analysis of different macromolecular crowding agents in human tenocyte cultures.Biomaterials and biosystems · 2026Article
- Pharmacological alternatives to oxytetracycline as potential treatment of flexural limb deformities in foals: a preliminary in vitro cell viability and proliferation study.Scientific reports · 2025Article
- State-of the-art and future perspective in co-culture systems for tendon engineering.Biomaterials and biosystems · 2025Article
- Article
- A decellularized flowable placental connective tissue matrix supports cellular functions of human tenocytes in vitro.Journal of experimental orthopaedics · 2022Article
- Dexamethasone Does not Compensate for Local Anesthetic Cytotoxic Effects on Tenocytes: Morphine or Morphine Plus Dexamethasone May Be a Safe Alternative.Arthroscopy, sports medicine, and rehabilitation · 2022Article
- Autograft Long Head Biceps Tendon Can Be Used as a Scaffold for Biologically Augmenting Rotator Cuff Repairs.Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association · 2022Article
- A Self-Powered Piezo-Bioelectric Device Regulates Tendon Repair-Associated Signaling Pathways through Modulation of Mechanosensitive Ion Channels.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- Impact of isolation method on cellular activation and presence of specific tendon cell subpopulations during in vitro culture.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Species variations in tenocytes' response to inflammation require careful selection of animal models for tendon research.Scientific reports · 2021Article
- Growth factor and macromolecular crowding supplementation in human tenocyte culture.Biomaterials and biosystems · 2021Article
- Identification and Distinction of Tenocytes and Tendon-Derived Stem Cells.Frontiers in cell and developmental biology · 2021Review
- Dose-Response Tendon-Specific Markers Induction by Growth Differentiation Factor-5 in Human Bone Marrow and Umbilical Cord Mesenchymal Stem Cells.International journal of molecular sciences · 2020Article
- Article
- Recovery of Tendon Characteristics by Inhibition of Aberrant Differentiation of Tendon-Derived Stem Cells from Degenerative Tendinopathy.International journal of molecular sciences · 2020Article
- Tendinosis develops from age- and oxygen tension-dependent modulation of Rac1 activity.Aging cell · 2019Article
- Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions.International journal of nanomedicine · 2018Article
- Tenogenic modulating insider factor: Systematic assessment on the functions of tenomodulin gene.Gene · 2016Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe treatment of rotator cuff tears is still challenging. Tendon tissue engineering (TTE) might be an alternative in future. Tenocytes seem to be the most suitable cell type as they are easy to obtain and no differentiation in vitro is necessary. The aim of this study was to examine, if the long head of the biceps tendon (LHB) can deliver viable tenocytes for TTE. In this context, different isolation methods, such as enzymatic digestion (ED) and cell migration (CM), are investigated on differences in gene expression and cell morphology.
methodsSamples of the LHB were obtained from patients, who underwent surgery for primary shoulder arthroplasty. Using ED as isolation method, 0.2% collagenase I solution was used. Using CM as isolation method, small pieces of minced tendon were put into petri-dishes. After cell cultivation, RT-PCR was performed for collagen type I, collagen type III, decorin, tenascin-C, fibronectin, Scleraxis, tenomodulin, osteopontin and agreccan.
resultsThe total number of isolated cells, in relation to 1 g of native tissue, was 14 times higher using ED. The time interval for cell isolation was about 17 hours using ED and approximately 50 days using CM. Cell morphology in vitro was similar for both isolation techniques. Higher expression of collagen type I could be observed in tenocyte-like cell cultures (TLCC) using ED as isolation method (p < 0.05), however decorin expression was higher in TLCC using CM as isolation method (p < 0.05). Dedifferentiation potential seemed to be similar for both isolation techniques.
conclusionIn summary tenocyte-like cells can be obtained with both isolation methods (ED and CM) from the LHB. As no obvious disadvantage could be seen using ED, this method is more suitable for clinical use, as time for cell isolation is shorter and a remarkably higher number of cells can be obtained. However, both isolation methods can further be improved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.