Evidence map›Paper›PMID 22890845›Full record

ArticleMolecular and cellular biology2012

FoxO-dependent regulation of diacylglycerol kinase α gene expression.

Mónica Martínez-Moreno, Job García-Liévana, Denise Soutar, Pedro Torres-Ayuso, Elena Andrada, Xiao-Ping Zhong, Gary A Koretzky, Isabel Mérida, Antonia Ávila-Flores

Open access · bronzeAbstract read
In one paragraph

Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Immune Checkpoint Receptors Signaling in T Cells.International journal of molecular sciences · 2022
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Review
  15. Review
  16. DGKα in Neutrophil Biology and Its Implications for Respiratory Diseases.International journal of molecular sciences · 2019
    Review
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Mónica Martínez-MorenoDepartment of Immunology and Oncology, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Job García-Liévana
Denise Soutar
Pedro Torres-Ayuso
Elena Andrada
Xiao-Ping Zhong
Gary A Koretzky
Isabel Mérida
Antonia Ávila-Flores
Centro Nacional de Biotecnología · ESConsejo Superior de Investigaciones Científicas · ESDuke Medical Center · USUniversity of Pennsylvania · US

Funding

Regulation of peripheral T cell toleranceR01AI079088 · NIAID · DUKE UNIVERSITY · PI ZHONG, XIAOPING · 2008 to 2019
$3.5M
TSC1-mTOR signaling and T cell toleranceR01AI101206 · NIAID · DUKE UNIVERSITY · PI ZHONG, XIAOPING · 2012 to 2016
$1.9M
A Murine Model of Chronic Autoimmune Hepatitis and Liver FibrosisR01AI076357 · NIAID · DUKE UNIVERSITY · PI ZHONG, XIAOPING · 2008 to 2012
$1.5M
NIAID NIH HHS R01 AI076357NIAID NIH HHS R01 AI079088NIAID NIH HHS R01 AI101206
6 · The paper itself

Abstract

Diacylglycerol kinase α (DGKα) regulates diacylglycerol levels, catalyzing its conversion into phosphatidic acid. The α isoform is central to immune response regulation; it downmodulates Ras-dependent pathways and is necessary for establishment of the unresponsive state termed anergy. DGKα functions are regulated in part at the transcriptional level although the mechanisms involved remain poorly understood. Here, we analyzed the 5' end structure of the mouse DGKα gene and detected three binding sites for forkhead box O (FoxO) transcription factors, whose function was confirmed using luciferase reporter constructs. FoxO1 and FoxO3 bound to the 5' regulatory region of DGKα in quiescent T cells, as well as after interleukin-2 (IL-2) withdrawal in activated T cells. FoxO binding to this region was lost after complete T cell activation or IL-2 addition, events that correlated with FoxO phosphorylation and a sustained decrease in DGKα gene expression. These data strongly support a role for FoxO proteins in promoting high DGKα levels and indicate a mechanism by which DGKα function is downregulated during productive T cell responses. Our study establishes a basis for a causal relationship between DGKα downregulation, IL-2, and anergy avoidance.

Indexed as

Gene Expression Regulation, EnzymologicAnimalsBinding SitesCell LineDiacylglycerol KinaseForkhead Box Protein O1Forkhead Box Protein O3Forkhead Transcription FactorsHumansInterleukin-2Lymphocyte ActivationMiceMice, Inbred BALB CMice, Inbred C57BLMice, TransgenicPhosphorylationDiacylglycerol KinaseForkhead Box Protein O1Forkhead Box Protein O3Forkhead Transcription FactorsFoxo1 protein, mouseFoxO3 protein, mouseInterleukin-2Receptors, Interleukin-2

Identifiers

PMID22890845
PMCPMC3457341
OpenAlexW2121844187

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.