Evidence map›Paper›PMID 22993231›Full record

ArticleJournal of lipid research2012

Intestine-specific expression of Apobec-1 rescues apolipoprotein B RNA editing and alters chylomicron production in Apobec1 -/- mice.

Valerie Blanc, Yan Xie, Jianyang Luo, Susan Kennedy, Nicholas O Davidson

Open access · hybridAbstract read
In one paragraph

Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Article
  5. From worms to humans: Understanding intestinal lipid metabolism via model organisms.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2023
    Review
  6. The Remnant Lipoprotein Hypothesis of Diabetes-Associated Cardiovascular Disease.Arteriosclerosis, thrombosis, and vascular biology · 2022
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Incorporating alternative splicing and mRNA editing into the genetic analysis of complex traits.BioEssays : news and reviews in molecular, cellular and developmental biology · 2014
    Article
  13. Article
  14. Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Valerie BlancDepartment of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
Yan Xie
Jianyang Luo
Susan Kennedy
Nicholas O Davidson
Washington University in St. Louis · US

Funding

Washington University DDRCC Supplemental Equipment RequestP30DK052574 · NIDDK · WASHINGTON UNIVERSITY · PI Nicholas O. Davidson · 2000 to 2026
$30.8M
HEPATIC SYNTHESIS AND SECRETION OF APOLIPOPROTEIN (A)R01DK056260 · NIDDK · WASHINGTON UNIVERSITY · PI DAVIDSON, NICHOLAS O. · 1999 to 2017
$5.9M
ENTEROHEPATIC LIPID FLUX AND APOPROTEIN BIOSYNTHESIS.R37HL038180 · NHLBI · WASHINGTON UNIVERSITY · PI DAVIDSON, NICHOLAS O. · 2000 to 2009
$5.1M
ENTEROHEPATIC LIPID FLUX AND APOPROTEIN BIOSYNTHESISR01HL038180 · NHLBI · WASHINGTON UNIVERSITY · PI DAVIDSON, NICHOLAS O. · 1986 to 2018
$3.8M
NHLBI NIH HHS HL-38180NHLBI NIH HHS R01 HL038180NHLBI NIH HHS R37 HL038180NIDDK NIH HHS DK-52574NIDDK NIH HHS DK-56260NIDDK NIH HHS P30 DK052574NIDDK NIH HHS R01 DK056260
6 · The paper itself

Abstract

Intestinal apolipoprotein B (apoB) mRNA undergoes C-to-U editing, mediated by the catalytic deaminase apobec-1, which results in translation of apoB48. Apobec1(-/-) mice produce only apoB100 and secrete larger chylomicron particles than those observed in wild-type (WT) mice. Here we show that transgenic rescue of intestinal apobec-1 expression (Apobec1(Int/O)) restores C-to-U RNA editing of apoB mRNA in vivo, including the canonical site at position 6666 and also at approximately 20 other newly identified downstream sites present in WT mice. The small intestine of Apobec1(Int/O) mice produces only apoB48, and the liver produces only apoB100. Serum chylomicron particles were smaller in Apobec1(Int/O) mice compared with those from Apobec1(-/-) mice, and the predominant fraction of serum apoB48 in Apobec1(Int/O) mice migrated in lipoproteins smaller than chylomicrons, even when these mice were fed a high-fat diet. Because apoB48 arises exclusively from the intestine in Apobec1(Int/O) mice and intestinal apoB48 synthesis and secretion rates were comparable to WT mice, we were able to infer the major sites of origin of serum apoB48 in WT mice. Our findings imply that less than 25% of serum apoB48 in WT mice arises from the intestine, with the majority originating from the liver.

Indexed as

RNA EditingAnimalsAPOBEC-1 DeaminaseApolipoproteins BChromatography, High Pressure LiquidChylomicronsCytidine DeaminaseIntestine, SmallMiceMice, Inbred C57BLMice, TransgenicMicroscopy, ElectronOrgan SpecificityRNA, MessengerAPOBEC-1 DeaminaseApobec1 protein, mouseApolipoproteins BChylomicronsCytidine DeaminaseRNA, Messenger

Identifiers

PMID22993231
PMCPMC3494256
OpenAlexW2112419967

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.