ArticleAmerican journal of respiratory cell and molecular biology2013
Tissue factor signals airway epithelial basal cell survival via coagulation and protease-activated receptor isoforms 1 and 2.
Article in American journal of respiratory cell and molecular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Factor 3 regulates airway engraftment by human bronchial basal cells.Stem cells translational medicine · 2025Article
- Manipulation of tissue factor-mediated basal PAR-2 signalling on macrophages determines sensitivity for IFNγ responsiveness and significantly modifies the phenotype of murine DTH.Frontiers in immunology · 2022Article
- Binding domain peptide ameliorates alveolar hypercoagulation and fibrinolytic inhibition in mice with lipopolysaccharide-induced acute respiratory distress syndrome Via NF-κB signaling pathway.American journal of translational research · 2022Article
- Lung Epithelial Cell Transcriptional Regulation as a Factor in COVID-19-associated Coagulopathies.American journal of respiratory cell and molecular biology · 2021Article
- Unique transcriptional changes in coagulation cascade genes in SARS-CoV-2-infected lung epithelial cells: A potential factor in COVID-19 coagulopathies.bioRxiv : the preprint server for biology · 2020Article
- Proteomic analysis reveals procoagulant properties of cigarette smoke-induced extracellular vesicles.Journal of extracellular vesicles · 2019Article
- Airway Progenitor Clone Formation Is Enhanced by Y-27632-Dependent Changes in the Transcriptome.American journal of respiratory cell and molecular biology · 2016Article
- Sulfur mustard inhalation: mechanisms of injury, alteration of coagulation, and fibrinolytic therapy.Annals of the New York Academy of Sciences · 2016Review
- Emerging targets for treating sulfur mustard-induced injuries.Annals of the New York Academy of Sciences · 2016Review
- Airway epithelium interactions with aeroallergens: role of secreted cytokines and chemokines in innate immunity.Frontiers in immunology · 2015Review
- In vitro cell culture model for toxic inhaled chemical testing.Journal of visualized experiments : JoVE · 2014Article
- Activation of protease-activated receptor 2 reduces glioblastoma cell apoptosis.Journal of biomedical science · 2014Article
- Human tracheobronchial basal cells. Normal versus remodeling/repairing phenotypes in vivo and in vitro.American journal of respiratory cell and molecular biology · 2013Article
- Stem cells, cell therapies, and bioengineering in lung biology and diseases. Comprehensive review of the recent literature 2010-2012.Annals of the American Thoracic Society · 2013Review
- Adenosine A2A receptor-dependent proliferation of pulmonary endothelial cells is mediated through calcium mobilization, PI3-kinase and ERK1/2 pathways.Biochemical and biophysical research communications · 2013Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Tissue factor (TF) initiates the extrinsic coagulation cascade and is a high-affinity receptor for coagulation factor VII. TF also participates in protease-activated receptor (PAR)1 and PAR2 activation. Human epithelial basal cells were previously purified on the basis of TF expression. The purpose of this study was to determine if tracheobronchial epithelial basal cell-associated TF drives coagulation and/or activates PARs to promote basal cell functions. We used human tracheobronchial tissues to isolate human airway epithelial cells using specific cell surface markers by flow cytometry and studied TF expression by immunostaining. TF-dependent fibrin network formation was observed by confocal and scanning electron microscopy. TF knockdown was done using short hairpin RNA, and TF mRNA was measured using quantitative RT-PCR. We found that 97 ± 5% of first-passage human tracheobronchial epithelial cells were basal cells, and 100% of these basal cells expressed TF. Basal cell-associated TF was active, but TF activity was dependent on added extrinsic coagulation cascade factors. TF inhibition caused basal cell apoptosis and necrosis. This was due to two parallel but interdependent TF-regulated processes: failure to generate a basal cell-associated fibrin network and suboptimal PAR1 and PAR2 activity. The data indicate that membrane surface TF mediates airway epithelial basal cell attachment, which maintains cell survival and mitotic potential. The implications of these findings are discussed in the context of basal cell-associated TF activity in normal and injured tissues and of the potential for repair of airway epithelium in lung disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.