ArticleJournal of immunology (Baltimore, Md. : 1950)2012
NK cell lytic granules are highly motile at the immunological synapse and require F-actin for post-degranulation persistence.
Article in Journal of immunology (Baltimore, Md. : 1950), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Short-Chain Fatty Acids at the Crossroads of Microbiota, Immunometabolism, and Inflammation.Biomedicines · 2026Review
- MINFLUX nanoscopy to study the NK cell immune synapse.Methods in cell biology · 2026Article
- Tumor cells escape immunosurveillance by hampering LFA-1.Frontiers in immunology · 2025Review
- β-Actin G342D as a Cause of NK Cell Deficiency Impairing Lytic Synapse Termination.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Locked and Loaded: Mechanisms Regulating Natural Killer Cell Lytic Granule Biogenesis and Release.Frontiers in immunology · 2022Review
- Inflammatory-driven NK cell maturation and its impact on pathology.Frontiers in immunology · 2022Review
- Cereblon regulates NK cell cytotoxicity and migration via Rac1 activation.European journal of immunology · 2021Article
- Degranulation enhances presynaptic membrane packing, which protects NK cells from perforin-mediated autolysis.PLoS biology · 2021Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors.Cellular & molecular immunology · 2020Article
- Molecular regulation of the plasma membrane-proximal cellular steps involved in NK cell cytolytic function.Journal of cell science · 2020Review
- Remodeling of secretory lysosomes during education tunes functional potential in NK cells.Nature communications · 2019Article
- Single Degranulations in NK Cells Can Mediate Target Cell Killing.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells.Current biology : CB · 2018Article
- Natural Killer Cells: Development, Maturation, and Clinical Utilization.Frontiers in immunology · 2018Review
- IQ Domain-Containing GTPase-Activating Protein 1 Regulates Cytoskeletal Reorganization and Facilitates NKG2D-Mediated Mechanistic Target of Rapamycin Complex 1 Activation and Cytokine Gene Translation in Natural Killer Cells.Frontiers in immunology · 2018Article
- Measurement of Lytic Granule Convergence After Formation of an NK Cell Immunological Synapse.Methods in molecular biology (Clifton, N.J.) · 2017Article
- Formin-generated actomyosin arcs propel T cell receptor microcluster movement at the immune synapse.The Journal of cell biology · 2016Article
- High- and Super-Resolution Microscopy Imaging of the NK Cell Immunological Synapse.Methods in molecular biology (Clifton, N.J.) · 2016Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The formation of a dynamic, actin-rich immunological synapse (IS) and the polarization of cytolytic granules toward target cells are essential to the cytotoxic function of NK cells. Following polarization, lytic granules navigate through the pervasive actin network at the IS to degranulate and secrete their toxic contents onto target cells. We examined lytic granule motility and persistence at the cell cortex of activated human NK cells, using high-resolution total internal reflection microscopy and highly quantitative analysis techniques. We illustrate that lytic granules are dynamic and observe substantial motility at the plane of the cell cortex prior to, but not after, degranulation. We also show that there is no significant change in granule motility in the presence of Latrunculin A (which induces actin depolymerization), when added after granule polarization, but that there is a significant decrease in lytic granule persistence subsequent to degranulation. Thus, we show that lytic granules are highly dynamic at the cytolytic human NK cell IS prior to degranulation and that the persistence of granules at the cortex following exocytosis requires the integrity of the synaptic actin network.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.