ArticlePlant cell reports2013
Accumulation of rice prolamin-GFP fusion proteins induces ER-derived protein bodies in transgenic rice calli.
Article in Plant cell reports, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Imaging the ER and Endomembrane System in Cereal Endosperm.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria.Transgenic research · 2020Article
- The localization of rice prolamin species in protein body type I is determined by the temporal control of gene expression of the respective prolamin promoters.Plant biotechnology (Tokyo, Japan) · 2018Article
- Specific region affects the difference in accumulation levels between apple food allergen Mal d 1 and birch pollen allergen Bet v 1 which are expressed in vegetative tissues of transgenic rice.Plant molecular biology · 2018Article
- Cellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain.International journal of molecular sciences · 2017Article
- Accumulation of foreign polypeptides to rice seed protein body type I using prolamin portion sequences.Plant cell reports · 2017Article
- Control of foreign polypeptide localization in specific layers of protein body type I in rice seed.Plant cell reports · 2016Article
- High CO2 concentration as an inductor agent to drive production of recombinant phytotoxic antimicrobial peptides in plant biofactories.Plant molecular biology · 2016Article
- The Encapsulation of Hemagglutinin in Protein Bodies Achieves a Stronger Immune Response in Mice than the Soluble Antigen.Frontiers in plant science · 2016Article
- The trafficking pathway of a wheat storage protein in transgenic rice endosperm.Annals of botany · 2014Article
- The Induction of Recombinant Protein Bodies in Different Subcellular Compartments Reveals a Cryptic Plastid-Targeting Signal in the 27-kDa γ-Zein Sequence.Frontiers in bioengineering and biotechnology · 2014Article
- The dynamic behavior of storage organelles in developing cereal seeds and its impact on the production of recombinant proteins.Frontiers in plant science · 2014Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageWe showed that rice prolamin polypeptides formed ER-derived PBs in transgenic rice calli, and that this heterologous transgene expression system is suitable for studying the mechanism of rice PB-I formation. Rice prolamins, alcohol-soluble seed storage proteins, accumulate directly within the rough endoplasmic reticulum (ER) lumen, leading to the formation of ER-derived type I protein bodies (PB-Is) in rice seed. Because rice prolamins do not possess a well-known ER retention signal such as K(H)DEL, or a unique sequence for retention in the ER such as a tandem repeat domain of maize and wheat prolamins, the mechanisms of prolamin accumulation in the ER and PB-I formation are poorly understood. In this study, we examined the formation mechanisms of PBs by expressing four types of rice prolamin species fused to green fluorescent protein (GFP) in transgenic rice calli. Each prolamin-GFP fusion protein was stably accumulated in rice calli and formed ER-derived PBs. In contrast, GFP fused with the signal peptide of prolamin was secreted into the intercellular space in rice calli. In addition, each of the four types of prolamin-GFP fusion proteins was co-localized with the ER chaperone binding protein. These results suggest that the mature polypeptide of prolamin is capable of being retained in the ER and induce the formation of PBs in non-seed tissue, and that the rice callus heterologous transgene expression system is useful for studying the mechanisms of rice PB-I formation.
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